Human nemo-like kinase ELISA Kit | NLK elisa kit

Human Serine/threonine-protein kinase NLK, NLK ELISA Kit

Cat. Num. AAA9336671

• Reactivity: Human
• Synonyms: nemo-like kinase; Human Serine/threonine-protein kinase NLK; NLK ELISA Kit; Human Serine/threonine-protein kinase NLK (NLK) ELISA kit; DKFZp761G1211; FLJ21033; nemo like kinase; NLK elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human NLK and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.25ng/ml-8ng/ml.
• Sensitivity: 0.1ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated NLK concentrations in Human serum, plasma and other body fluids. Using Purified Human NLK antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NLK and NLK antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NLK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for NLK elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated NLK concentrations in Human serum, plasma and other body fluids. Using Purified Human NLK antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NLK and NLK antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NLK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 149408126
• NCBI GeneID: 51701
• NCBI Accession #: NP_057315.3
• NCBI GenBank Nucleotide #: NM_016231.4
• UniProt Accession #: Q9UBE8; Q2PNI9; Q6P2A3; B2RCX1
• Molecular Weight: 58,283 Da
• NCBI Official Full Name: serine/threonine-protein kinase NLK
• NCBI Official Synonym Full Names: nemo-like kinase
• NCBI Official Symbol: NLK
• NCBI Protein Information: serine/threonine-protein kinase NLK
• UniProt Protein Name: Serine/threonine-protein kinase NLK
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: NLK
• UniProt Synonym Gene Names: LAK1
• UniProt Entry Name: NLK_HUMAN

Uniprot Description

NLK: Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK- SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Protein type: Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.24; CMGC group; MAPK family; nmo subfamily

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; nucleus; cytosol

Molecular Function: MAP kinase activity; protein serine/threonine kinase activity; protein binding; ubiquitin protein ligase binding; magnesium ion binding; SH2 domain binding; transcription factor binding; ATP binding; protein kinase activity

Biological Process: Wnt receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; regulation of transcription, DNA-dependent; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein amino acid autophosphorylation; peptidyl-threonine phosphorylation; MAPKKK cascade; protein amino acid phosphorylation; serine phosphorylation of STAT3 protein