Human neutral sphingomyelinase (N-SMase) activation associated factor ELISA Kit | NSMAF elisa kit

Human Protein FAN, NSMAF ELISA Kit

Cat. Num. AAA9335966

• Gene Names: NSMAF; FAN
• Reactivity: Human
• Synonyms: neutral sphingomyelinase (N-SMase) activation associated factor; Human Protein FAN; NSMAF ELISA Kit; Human Protein FAN (NSMAF) ELISA kit; FAN; ; NSMAF elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human NSMAF and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.25 ng/ml - 8 ng/ml.
• Sensitivity: 0.1 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated NSMAF concentrations in Human serum, plasma and other body fluids. Using Purified Human NSMAF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NSMAF and NSMAF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NSMAF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for NSMAF elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated NSMAF concentrations in Human serum, plasma and other body fluids. Using Purified Human NSMAF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NSMAF and NSMAF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NSMAF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 221625547
• NCBI GeneID: 8439
• NCBI Accession #: NP_001138244.1
• NCBI GenBank Nucleotide #: NM_001144772.1
• UniProt Accession #: Q92636; Q8IW26; B4DFB0; E9PCH0
• Molecular Weight: 104,372 Da
• NCBI Official Full Name: protein FAN isoform 2
• NCBI Official Synonym Full Names: neutral sphingomyelinase (N-SMase) activation associated factor
• NCBI Official Symbol: NSMAF
• NCBI Official Synonym Symbols: FAN
• NCBI Protein Information: protein FAN; factor associated with N-SMase activation; factor associated with neutral sphingomyelinase activation
• UniProt Protein Name: Protein FAN
• Protein Family: Protein
• UniProt Gene Name: NSMAF
• UniProt Synonym Gene Names: FAN; Factor associated with N-SMase activation
• UniProt Entry Name: FAN_HUMAN

NCBI Description

This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]

Uniprot Description

NSMAF: Couples the p55 TNF-receptor (TNF-R55 / TNFR1) to neutral sphingomyelinase (N-SMASE). Specifically binds to the N- smase activation domain of TNF-R55. May regulate ceramide production by N-SMASE. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Autophagy

Chromosomal Location of Human Ortholog: 8q12-q13

Cellular Component: extrinsic to membrane; cytoplasm; endomembrane system

Molecular Function: sphingomyelin phosphodiesterase activator activity; protein binding; receptor signaling protein activity; phospholipid binding

Biological Process: positive regulation of catalytic activity; positive regulation of apoptosis; ceramide metabolic process; endosome transport; signal transduction