Typical Testing Data/Standard Curve (for reference only)
Human Neutral Sphingomyelinase Activation Associated Factor (NSMAF) ELISA Kit | NSMAF elisa kit
Human Neutral Sphingomyelinase Activation Associated Factor (NSMAF) ELISA Kit
Gene Names
NSMAF; FAN
Reactivity
Human
Synonyms
Neutral Sphingomyelinase Activation Associated Factor (NSMAF); Human Neutral Sphingomyelinase Activation Associated Factor (NSMAF) ELISA Kit; FAN; Factor associated with neutral sphingomyelinase activation; NSMAF elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Neutral Sphingomyelinase Activation Associated Factor (NSMAF).
No significant cross-reactivity or interference between Neutral Sphingomyelinase Activation Associated Factor (NSMAF) and analogues was observed.
No significant cross-reactivity or interference between Neutral Sphingomyelinase Activation Associated Factor (NSMAF) and analogues was observed.
Sequence Length
948
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.054ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Neutral Sphingomyelinase Activation Associated Factor (NSMAF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Neutral Sphingomyelinase Activation Associated Factor (NSMAF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NSMAF elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutral Sphingomyelinase Activation Associated Factor (NSMAF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neutral Sphingomyelinase Activation Associated Factor (NSMAF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neutral Sphingomyelinase Activation Associated Factor (NSMAF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neutral Sphingomyelinase Activation Associated Factor (NSMAF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for NSMAF elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
107,314 Da
NCBI Official Full Name
Neutral sphingomyelinase (N-SMase) activation associated factor
NCBI Official Synonym Full Names
neutral sphingomyelinase (N-SMase) activation associated factor
NCBI Official Symbol
NSMAF
NCBI Official Synonym Symbols
FAN
NCBI Protein Information
protein FAN
UniProt Protein Name
Protein FAN
Protein Family
UniProt Gene Name
NSMAF
UniProt Synonym Gene Names
FAN; Factor associated with N-SMase activation
UniProt Entry Name
FAN_HUMAN
NCBI Description
This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]
Uniprot Description
NSMAF: Couples the p55 TNF-receptor (TNF-R55 / TNFR1) to neutral sphingomyelinase (N-SMASE). Specifically binds to the N- smase activation domain of TNF-R55. May regulate ceramide production by N-SMASE. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold; Autophagy
Chromosomal Location of Human Ortholog: 8q12-q13
Cellular Component: extrinsic to membrane; cytoplasm; endomembrane system
Molecular Function: sphingomyelin phosphodiesterase activator activity; protein binding; receptor signaling protein activity; phospholipid binding
Biological Process: positive regulation of catalytic activity; positive regulation of apoptosis; ceramide metabolic process; signal transduction; endosome transport
Research Articles on NSMAF
Similar Products
Product Notes
The Human NSMAF nsmaf (Catalog #AAA2024999) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2024999 ELISA Kit recognizes Human NSMAF. It is sometimes possible for the material contained within the vial of "Neutral Sphingomyelinase Activation Associated Factor (NSMAF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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