Human forkhead box N1 ELISA Kit | FOXN1 elisa kit

Human Forkhead box protein N1, FOXN1 ELISA Kit

Cat. Num. AAA9330404

• Gene Names: FOXN1; WHN; RONU; FKHL20
• Reactivity: Human
• Synonyms: forkhead box N1; Human Forkhead box protein N1; FOXN1 ELISA Kit; Human Forkhead box protein N1 (FOXN1) ELISA kit; FKHL20; RONU; WHN; Rowett nude; winged helix nude; winged-helix nude; FOXN1 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human FOXN1 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-1000pg/ml.
• Sensitivity: 5.0pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated FOXN1 concentrations in Human serum, plasma and other body fluids. Using Purified Human FOXN1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FOXN1 and FOXN1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FOXN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for FOXN1 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated FOXN1 concentrations in Human serum, plasma and other body fluids. Using Purified Human FOXN1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FOXN1 and FOXN1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FOXN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18201913
• NCBI GeneID: 8456
• NCBI Accession #: NP_003584.2
• NCBI GenBank Nucleotide #: NM_003593.2
• UniProt Accession #: O15353; O15352; B2R9Q7
• Molecular Weight: 68,925 Da
• NCBI Official Full Name: forkhead box protein N1
• NCBI Official Synonym Full Names: forkhead box N1
• NCBI Official Symbol: FOXN1
• NCBI Official Synonym Symbols: WHN; RONU; FKHL20
• NCBI Protein Information: forkhead box protein N1; Rowett nude; winged helix nude; winged-helix nude; winged-helix transcription factor nude
• UniProt Protein Name: Forkhead box protein N1
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXN1
• UniProt Synonym Gene Names: RONU; WHN
• UniProt Entry Name: FOXN1_HUMAN

NCBI Description

Mutations in the winged-helix transcription factor gene at the nude locus in mice and rats produce the pleiotropic phenotype of hairlessness and athymia, resulting in a severely compromised immune system. This gene is orthologous to the mouse and rat genes and encodes a similar DNA-binding transcription factor that is thought to regulate keratin gene expression. A mutation in this gene has been correlated with T-cell immunodeficiency, the skin disorder congenital alopecia, and nail dystrophy. Alternative splicing in the 5' UTR of this gene has been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXN1: Transcriptional regulator involved in development. Defects in FOXN1 are the cause of T-cell immunodeficiency congenital alopecia and nail dystrophy (TIDAND). A disorder characterized by the association of congenital alopecia, severe T-cell immunodeficiency, and ridging and pitting of all nails.

Protein type: Transcription factor; DNA-binding; Cell development/differentiation

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleus

Molecular Function: sequence-specific DNA binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; keratinocyte differentiation; epidermis development; organ morphogenesis; epithelial cell proliferation; hair follicle development; thymus development; regulation of T cell differentiation in the thymus; defense response; lymphocyte homeostasis

Disease: T-cell Immunodeficiency, Congenital Alopecia, And Nail Dystrophy