Human methyl-CpG binding domain protein 4 ELISA Kit | MBD4 elisa kit

Human Methyl-CpG-binding domain protein 4, MBD4 ELISA Kit

Cat. Num. AAA9321203

• Gene Names: MBD4; MED1
• Reactivity: Human
• Synonyms: methyl-CpG binding domain protein 4; Human Methyl-CpG-binding domain protein 4; MBD4 ELISA Kit; Human Methyl-CpG-binding domain protein 4 (MBD4) ELISA kit; MED1; 3; N (4) -ethenocytosine glycosylase; G/5-fluorouracil mismatch glycosylase with biphasic kinetics; G/T mismatch glycosylase; G/U mismatch glycosylase; putative methyl-CpG binding protein; MBD4 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between this analyte and analogues is observed.

• Samples: Undiluted original Human body fluids, tissue homogenates, secretions or feces samples.
• Assay Type: Sandwich (Quantitative)
• Detection Range: 3.12ng/ml-100ng/ml.
• Sensitivity: 1.0ng/ml.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for MBD4 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of MBD4 (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 442796450
• NCBI GeneID: 8930
• NCBI Accession #: NP_001263199.1
• NCBI GenBank Nucleotide #: NM_001276270.1
• UniProt Accession #: O95243; Q2MD36; Q7Z4T3; Q96F09; B4DZN2; D3DNC3; D3DNC4; E9PEE4
• Molecular Weight: 66,051 Da
• NCBI Official Full Name: methyl-CpG-binding domain protein 4 isoform 2
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 4
• NCBI Official Symbol: MBD4
• NCBI Official Synonym Symbols: MED1
• NCBI Protein Information: methyl-CpG-binding domain protein 4; G/T mismatch glycosylase; G/U mismatch glycosylase; methyl-CpG-binding protein MBD4; 3,N(4)-ethenocytosine glycosylase; methyl-CpG-binding endonuclease 1; mismatch-specific DNA N-glycosylase; putative methyl-CpG binding protein; G/5-fluorouracil mismatch glycosylase with biphasic kinetics
• UniProt Protein Name: Methyl-CpG-binding domain protein 4
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: MBD4
• UniProt Synonym Gene Names: MED1
• UniProt Entry Name: MBD4_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains an MBD domain at the N-terminus that functions both in binding to methylated DNA and in protein interactions and a C-terminal mismatch-specific glycosylase domain that is involved in DNA repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]

Uniprot Description

MBD4: Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Deoxyribonuclease; EC 3.2.2.-; DNA repair, damage; Apoptosis

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: nucleoplasm; cytoplasm; nucleus; chromatin

Molecular Function: protein binding; satellite DNA binding; pyrimidine-specific mismatch base pair DNA N-glycosylase activity; endodeoxyribonuclease activity

Biological Process: response to radiation; base-excision repair, AP site formation; DNA damage response, signal transduction resulting in induction of apoptosis; base-excision repair; depyrimidination; mitotic cell cycle G2/M transition DNA damage checkpoint; DNA repair; DNA catabolic process, endonucleolytic