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Typical Testing Data/Standard Curve (for reference only)

Human tumor necrosis factor receptor superfamily, member 18 ELISA Kit | TNFRSF18 elisa kit

Human Tumor necrosis factor receptor superfamily member 18, TNFRSF18 ELISA Kit

Gene Names
TNFRSF18; AITR; GITR; CD357; GITR-D
Reactivity
Human
Synonyms
tumor necrosis factor receptor superfamily; member 18; Human Tumor necrosis factor receptor superfamily member 18; TNFRSF18 ELISA Kit; Human Tumor necrosis factor receptor superfamily member 18 (TNFRSF18) ELISA kit; AITR; GITR; GITR-D; TNF receptor superfamily activation-inducible protein; activation-inducible TNFR family receptor; glucocorticoid-induced TNFR-related protein; TNFRSF18 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human TNFRSF18. No significant cross-reactivity or interference between Human TNFRSF18 and analogues was observed.
Sequence Length
241
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
0.625 ng/ml-40 ng/ml
Sensitivity
0.156 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Apoptosis/Autophagy
Protein Biological Process 3
Apoptosis
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TNFRSF18 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TNFRSF18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFRSF18 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF18 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF18 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26,000 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 18 isoform 1
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 18
NCBI Official Symbol
TNFRSF18
NCBI Official Synonym Symbols
AITR; GITR; CD357; GITR-D
NCBI Protein Information
tumor necrosis factor receptor superfamily member 18; activation-inducible TNFR family receptor; glucocorticoid-induced TNFR-related protein; TNF receptor superfamily activation-inducible protein
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 18
UniProt Gene Name
TNFRSF18
UniProt Synonym Gene Names
AITR; GITR; UNQ319/PRO364
UniProt Entry Name
TNR18_HUMAN

NCBI Description

This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Feb 2011]

Uniprot Description

TNFRSF18: Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, cytokine; Apoptosis

Chromosomal Location of Human Ortholog: 1p36.3

Cellular Component: integral to plasma membrane; extracellular region; external side of plasma membrane

Molecular Function: tumor necrosis factor receptor activity

Biological Process: tumor necrosis factor-mediated signaling pathway; apoptosis; signal transduction; negative regulation of apoptosis

Research Articles on TNFRSF18

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Product Notes

The Human TNFRSF18 tnfrsf18 (Catalog #AAA930939) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA930939 ELISA Kit recognizes Human TNFRSF18. It is sometimes possible for the material contained within the vial of "tumor necrosis factor receptor superfamily, member 18, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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