Human serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1) , member 2 ELISA Kit | SERPINE2 elisa kit

Human Glia-derived nexin, SERPINE2 ELISA Kit

Cat. Num. AAA927777

• Gene Names: SERPINE2; GDN; PI7; PN1; PNI; PI-7; PN-1; GDNPF
• Reactivity: Human
• Synonyms: serpin peptidase inhibitor; clade E (nexin; plasminogen activator inhibitor type 1); member 2; Human Glia-derived nexin; SERPINE2 ELISA Kit; Human Glia-derived nexin (SERPINE2) ELISA kit; GDN; PI7; PN1; PNI; OTTHUMP00000203766; glial-derived neurite promoting factor; glial-derived nexin 1; plasminogen activator inhibitor type 1; protease inhibitor 7 (protease nexin I) ; serine (o; SERPINE2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human SERPINE2. No significant cross-reactivity or interference between human SERPINE2 and analogues was observed.
• Sequence Length: 397

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312 ng/ml-20 ng/ml
• Sensitivity: < 0.078 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SERPINE2 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SERPINE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPINE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINE2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 211904152
• NCBI GeneID: 5270
• NCBI Accession #: NP_001130000.1
• NCBI GenBank Nucleotide #: NM_001136528.1
• UniProt Accession #: P07093; Q53S15; Q5D0C4; B2R6A4; B4DIF2
• Molecular Weight: 44,002 Da
• NCBI Official Full Name: glia-derived nexin isoform b
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
• NCBI Official Symbol: SERPINE2
• NCBI Official Synonym Symbols: GDN; PI7; PN1; PNI; PI-7; PN-1; GDNPF
• NCBI Protein Information: glia-derived nexin; serpin E2; protease nexin I; peptidase inhibitor 7; glial-derived neurite promoting factor
• UniProt Protein Name: Glia-derived nexin
• Protein Family: Glia-derived nexin
• UniProt Gene Name: SERPINE2
• UniProt Synonym Gene Names: PI7; PN1; GDN; PI-7; PN-1
• UniProt Entry Name: GDN_HUMAN

NCBI Description

This gene encodes a member of the serpin family of proteins, a group of proteins that inhibit serine proteases. Thrombin, urokinase, plasmin and trypsin are among the proteases that this family member can inhibit. This gene is a susceptibility gene for chronic obstructive pulmonary disease and for emphysema. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

SERPINE2: Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: extracellular matrix; extracellular space; extrinsic to external side of plasma membrane; extracellular region; cytosol; neuromuscular junction; vesicle

Molecular Function: heparin binding; serine-type endopeptidase inhibitor activity; protein binding; glycosaminoglycan binding; receptor binding

Biological Process: negative regulation of proteolysis; regulation of synaptic transmission, glutamatergic; secretory granule organization and biogenesis; cerebellar granular layer morphogenesis; negative regulation of blood coagulation; detection of mechanical stimulus involved in sensory perception; mating plug formation; regulation of cell migration; negative regulation of cell proliferation; negative regulation of smoothened signaling pathway; negative regulation of cell growth; negative regulation of protein catabolic process; blood coagulation; regulation of timing of cell differentiation; negative regulation of phosphoinositide 3-kinase cascade; secretion by cell; positive regulation of astrocyte differentiation