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Typical Testing Data/Standard Curve (for reference only)

Mouse serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1) , member 2 ELISA Kit | SERPINE2 elisa kit

Mouse Glia-derived nexin, SERPINE2 ELISA Kit

Gene Names
Serpine2; PI7; PI-7; PN-1; Spi4; PAI-1; B230326M24Rik
Reactivity
Mouse
Synonyms
serpin peptidase inhibitor; clade E (nexin; plasminogen activator inhibitor type 1); member 2; Mouse Glia-derived nexin; SERPINE2 ELISA Kit; Mouse Glia-derived nexin (SERPINE2) ELISA kit; GDN; PI7; PN1; PNI; OTTHUMP00000203766; glial-derived neurite promoting factor; glial-derived nexin 1; plasminogen activator inhibitor type 1; protease inhibitor 7 (protease nexin I) ; serine (o; SERPINE2 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse SERPINE2. No significant cross-reactivity or interference between mouse SERPINE2 and analogues was observed.
Sequence Length
397
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
3.12 pg/ml-200 pg/ml
Sensitivity
< 0.78 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SERPINE2 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SERPINE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPINE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINE2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,207 Da
NCBI Official Full Name
glia-derived nexin
NCBI Official Synonym Full Names
serine (or cysteine) peptidase inhibitor, clade E, member 2
NCBI Official Symbol
Serpine2
NCBI Official Synonym Symbols
PI7; PI-7; PN-1; Spi4; PAI-1; B230326M24Rik
NCBI Protein Information
glia-derived nexin; GDN; serpin E2; protease nexin 1; protease nexin I; peptidase inhibitor 7; serine protease inhibitor 4; serine protease-inhibitor 4; serine (or cysteine) proteinase inhibitor, clade E, member 2; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
UniProt Protein Name
Glia-derived nexin
Protein Family
UniProt Gene Name
Serpine2
UniProt Synonym Gene Names
Pi7; Pn1; Spi4; GDN; PI-7; PN-1
UniProt Entry Name
GDN_MOUSE

Uniprot Description

SERPINE2: Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Inhibitor; Secreted, signal peptide

Cellular Component: extracellular matrix; extracellular space; extrinsic to external side of plasma membrane; membrane; cell soma; cell; extracellular region; cytosol; neuromuscular junction; vesicle

Molecular Function: serine-type endopeptidase inhibitor activity; heparin binding; glycosaminoglycan binding; protease inhibitor activity; receptor binding

Biological Process: negative regulation of proteolysis; nervous system development; regulation of synaptic transmission, glutamatergic; secretory granule organization and biogenesis; multicellular organismal development; cerebellar granular layer morphogenesis; negative regulation of peptidase activity; negative regulation of blood coagulation; mating plug formation; detection of mechanical stimulus involved in sensory perception; negative regulation of cell proliferation; negative regulation of smoothened signaling pathway; response to wounding; negative regulation of cell growth; blood coagulation; cell differentiation; negative regulation of protein catabolic process; regulation of timing of cell differentiation; negative regulation of phosphoinositide 3-kinase cascade; positive regulation of astrocyte differentiation; secretion by cell

Research Articles on SERPINE2

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Product Notes

The Mouse SERPINE2 serpine2 (Catalog #AAA928069) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA928069 ELISA Kit recognizes Mouse SERPINE2. It is sometimes possible for the material contained within the vial of "serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1) , member 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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