Immunofluorescence (IF)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Pdx1 with AP12326C at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. The nuclear counter stain is DAPI (blue).)
Rabbit DVL1 Polyclonal Antibody | anti-DVL1 antibody
DVL1 Antibody (Center)
Predicted: Rat
Predicted: Rat
WB: 1:2000
Immunofluorescence (IF)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Pdx1 with AP12326C at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. The nuclear counter stain is DAPI (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Pdx1 with AP12326C at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. The nuclear counter stain is DAPI (blue).)
Western Blot (WB)
(All lanes : Anti-DVL1 Antibody (Center) at 1:2000 dilutionLane 1: NIH/3T3 whole cell lysateLane 2: PC-3 whole cell lysateLane 3: K562 whole cell lysateLane 4: MDA-MB453 whole cell lysateLane 5: human kidney lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-DVL1 Antibody (Center) at 1:2000 dilutionLane 1: NIH/3T3 whole cell lysateLane 2: PC-3 whole cell lysateLane 3: K562 whole cell lysateLane 4: MDA-MB453 whole cell lysateLane 5: human kidney lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Background: DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation, acting as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 is a candidate gene for neuroblastomatous transformation. The Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1. The phenotypes of these diseases may be consistent with defects which might be expected from aberrant expression of a DVL gene during development.
NCBI and Uniprot Product Information
NCBI Description
DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation, acting as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 is a candidate gene for neuroblastomatous transformation. The Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1. The phenotypes of these diseases may be consistent with defects which might be expected from aberrant expression of a DVL gene during development. [provided by RefSeq, Jul 2008]
Uniprot Description
DVL1: Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Required for LEF1 activation upon WNT1 and WNT3A signaling. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Belongs to the DSH family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Inhibitor
Chromosomal Location of Human Ortholog: 1p36
Cellular Component: microtubule; growth cone; cell soma; clathrin-coated vesicle; axon; cytoplasm; cytoplasmic membrane-bound vesicle; dendrite; synapse; cytoplasmic vesicle; cytosol; lateral plasma membrane
Molecular Function: identical protein binding; protein binding; enzyme binding; frizzled binding; Rac GTPase binding; protein kinase binding
Biological Process: transcription from RNA polymerase II promoter; neural tube development; axon guidance; Wnt receptor signaling pathway; prepulse inhibition; convergent extension involved in neural plate elongation; axon extension; Wnt receptor signaling pathway, planar cell polarity pathway; dendrite morphogenesis; neurotransmitter secretion; regulation of neurotransmitter levels; heart development; positive regulation of transcription, DNA-dependent; Wnt receptor signaling pathway through beta-catenin; social behavior; receptor clustering; cytoplasmic microtubule organization and biogenesis; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; collateral sprouting; synapse organization and biogenesis; negative regulation of protein kinase activity; negative regulation of protein binding; positive regulation of Wnt receptor signaling pathway; neuromuscular junction development
Disease: Robinow Syndrome, Autosomal Dominant; Robinow Syndrome, Autosomal Dominant 2
Research Articles on DVL1
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Product Notes
The DVL1 dvl1p1 (Catalog #AAA9232183) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DVL1 Antibody (Center) reacts with Human, Mouse Predicted: Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DVL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). IF: 1:25 WB: 1:2000. Researchers should empirically determine the suitability of the DVL1 dvl1p1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DVL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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