Mouse anti-Human MAC-2 / Galectin 3 Monoclonal Antibody | anti-LGALS3 antibody

Mouse Monoclonal [clone 1G8] (IgG1) to Human MAC-2 / Galectin 3

Cat. Num. AAA249020

• Gene Names: LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Protein A/G Purified
• Synonyms: MAC-2 / Galectin 3; Monoclonal Antibody; Mouse Monoclonal [clone 1G8] (IgG1) to Human MAC-2 / Galectin 3; Anti-MAC-2 / Galectin 3 Antibody (clone 1G8) IHC-plus; 35 kd lectin; 35 kDa lectin; CBP35; GAL3; Galactoside-binding protein; GALIG; IgE-binding protein; Laminin-binding protein; Gal-3; GALBP; Mac-2 antigen; L31; MAC2; CBP 35; Galactose-specific lectin 3; Galectin-3; L-31; Lectin L-29; Galectin 3; Human MAC-2; anti-LGALS3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1G8
• Specificity: Human MAC-2 / Galectin-3
• Purity/Purification: Protein A/G Purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 250

• Applicable Applications for anti-LGALS3 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF (1:100), IHC-P (10 ug/ml), WB (1:200 - 1:4000)
• Target Species: Human
• Immunogen Description: Full length human recombinant protein of human LGALS3(NP_002297) produced in HEK293T cell.
• Immunogen Type: Recombinant protein
• Immunogen: MAC-2 / Galectin 3 antibody was raised against full length human recombinant protein of human LGALS3(NP_002297) produced in HEK293T cell.
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-LGALS3 / Galectin 3 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunofluorescence (IF)

(Immunofluorescent staining of HeLa cells using anti-LGALS3 mouse monoclonal antibody.)

Western Blot (WB)

(Western blot of extracts (35 ug) from 9 different cell lines by using anti-LGALS3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).)

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3.)

NCBI and Uniprot Product Information

• NCBI GI #: 115430223
• NCBI GeneID: 3958
• NCBI Accession #: NP_002297.2
• NCBI GenBank Nucleotide #: NM_002306.3
• UniProt Accession #: P17931; Q16005; Q6IBA7; Q96J47; B2RC38
• Molecular Weight: 26,152 Da
• NCBI Official Full Name: galectin-3 isoform 1
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 3
• NCBI Official Symbol: LGALS3
• NCBI Official Synonym Symbols: L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
• NCBI Protein Information: galectin-3; lectin L-29; 35 kDa lectin; MAC-2 antigen; IgE-binding protein; laminin-binding protein; galactose-specific lectin 3; carbohydrate-binding protein 35
• UniProt Protein Name: Galectin-3
• UniProt Gene Name: LGALS3
• UniProt Synonym Gene Names: MAC2; Gal-3; CBP 35; GALBP
• UniProt Entry Name: LEG3_HUMAN

NCBI Description

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]

Uniprot Description

Galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages.

Protein type: Extracellular matrix; Cell surface; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: extracellular matrix; spliceosome; extracellular space; proteinaceous extracellular matrix; membrane; mitochondrial inner membrane; cytoplasm; plasma membrane; immunological synapse; nucleus; external side of plasma membrane

Molecular Function: protein binding; laminin binding; IgE binding; carbohydrate binding; chemoattractant activity

Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; RNA splicing; negative regulation of endocytosis; regulation of T cell proliferation; negative regulation of T cell receptor signaling pathway; macrophage chemotaxis; monocyte chemotaxis; positive chemotaxis; epithelial cell differentiation; eosinophil chemotaxis; innate immune response; mRNA processing; skeletal development