Western Blot (WB)
(All lanes : Anti-Suz12 Antibody (Center) at 1:1000 dilutionLane 1: F9 whole cell lysatesLane 2: SW480 whole cell lysatesLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 83 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, Mouse Suz12 Polyclonal Antibody | anti-Suz12 antibody
(Mouse) Suz12 Antibody (Center)
Gene Names
Suz12; AI195385; AU016842; AW536442; mKIAA0160; D11Ertd530e; 2610028O16Rik
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Synonyms
Suz12; Polyclonal Antibody; (Mouse) Suz12 Antibody (Center); Polycomb protein Suz12; Suppressor of zeste 12 protein homolog; D11Ertd530e; Kiaa0160; anti-Suz12 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Ig
Specificity
This Mouse Suz12 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 496-530 amino acids from the Central region of Mouse Suz12.
Sequence Length
718
Applicable Applications for anti-Suz12 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000
Antigen Source
Human
Antigen Region
496-530
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(All lanes : Anti-Suz12 Antibody (Center) at 1:1000 dilutionLane 1: F9 whole cell lysatesLane 2: SW480 whole cell lysatesLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 83 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-Suz12 Antibody (Center) at 1:1000 dilutionLane 1: F9 whole cell lysatesLane 2: SW480 whole cell lysatesLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 83 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-Suz12 antibody
Description: Purified Rabbit Polyclonal Antibody (Pab)
Background: Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (By similarity). Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8.
Background: Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (By similarity). Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8.
References
Okazaki N.,et al.DNA Res. 10:35-48(2003). Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009). Pasini D.,et al.EMBO J. 23:4061-4071(2004). Umlauf D.,et al.Nat. Genet. 36:1296-1300(2004). Kuzmichev A.,et al.Proc. Natl. Acad. Sci. U.S.A. 102:1859-1864(2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
83026
NCBI Official Full Name
polycomb protein Suz12 isoform 2
NCBI Official Synonym Full Names
SUZ12 polycomb repressive complex 2 subunit
NCBI Official Symbol
Suz12
NCBI Official Synonym Symbols
AI195385; AU016842; AW536442; mKIAA0160; D11Ertd530e; 2610028O16Rik
NCBI Protein Information
polycomb protein Suz12
UniProt Protein Name
Polycomb protein Suz12
Protein Family
UniProt Gene Name
Suz12
UniProt Synonym Gene Names
D11Ertd530e; Kiaa0160
UniProt Entry Name
SUZ12_MOUSE
NCBI Description
This gene encodes a core component of the polycomb repressive complex 2 (PRC2) that also includes, at least, embryonic ectoderm development protein (EED) and enhancer of zeste homolog 1 or 2 (EZH1 or EZH2). Through the methyltransferase activity of EZH1 or EZH2, the PRC2 complex methylates Lys9 and Lys27 of histone 3 and Lys26 of histone 1, leading to recruitment of the PRC1 complex, histone 2A ubiquitylation and transcriptional repression of the target genes. This gene product is essential for the activity and integrity of the PRC2 complex, and is required for X chromosome inactivation, stem cell maintenance and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]
Research Articles on Suz12
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Product Notes
The Suz12 suz12 (Catalog #AAA9232061) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The (Mouse) Suz12 Antibody (Center) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Suz12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000. Researchers should empirically determine the suitability of the Suz12 suz12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Suz12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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