Western Blot (WB)
(All lanes : Anti-Suz12 Antibody (Center) at 1:1000 dilutionLane 1: F9 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: MCF-7 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 83 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit Suz12 Polyclonal Antibody | anti-Suz12 antibody
(Mouse) Suz12 Antibody (Center)
Western Blot (WB)
(All lanes : Anti-Suz12 Antibody (Center) at 1:1000 dilutionLane 1: F9 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: MCF-7 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 83 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-Suz12 Antibody (Center) at 1:1000 dilutionLane 1: F9 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: MCF-7 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 83 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western blot analysis of lysate from mouse F9 cell line, using Suz12 Antibody (Center). MBS9205759 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Western Blot (WB)
(Western blot analysis of lysate from mouse F9 cell line, using Suz12 Antibody (Center). MBS9205759 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009).
Pasini D.,et al.EMBO J. 23:4061-4071(2004).
Umlauf D.,et al.Nat. Genet. 36:1296-1300(2004).
Kuzmichev A.,et al.Proc. Natl. Acad. Sci. U.S.A. 102:1859-1864(2005).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a core component of the polycomb repressive complex 2 (PRC2) that also includes, at least, embryonic ectoderm development protein (EED) and enhancer of zeste homolog 1 or 2 (EZH1 or EZH2). Through the methyltransferase activity of EZH1 or EZH2, the PRC2 complex methylates Lys9 and Lys27 of histone 3 and Lys26 of histone 1, leading to recruitment of the PRC1 complex, histone 2A ubiquitylation and transcriptional repression of the target genes. This gene product is essential for the activity and integrity of the PRC2 complex, and is required for X chromosome inactivation, stem cell maintenance and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]
Uniprot Description
SUZ12: a zinc finger protein. A breakpoint translocation of the cognate gene with JAZF1 has been reported in endometrial stromal sarcoma.
Protein type: Oncoprotein; Transcription factor; C2H2-type zinc finger protein
Cellular Component: sex chromatin; ESC/E(Z) complex; nucleolus; chromosome; nucleus
Molecular Function: histone methyltransferase activity; protein binding; RNA binding; sequence-specific DNA binding; metal ion binding; chromatin binding; methylated histone residue binding
Biological Process: negative regulation of chemokine production; histone methylation; negative regulation of cell differentiation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; histone ubiquitination; positive regulation of cell proliferation; negative regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; negative regulation of epithelial cell proliferation
Research Articles on Suz12
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Product Notes
The Suz12 suz12 (Catalog #AAA9205759) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The (Mouse) Suz12 Antibody (Center) reacts with Mouse (Predicted Reactivity: Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Suz12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Suz12 suz12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Suz12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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