Western Blot (WB)
(All lanes : Anti-TPI1 Antibody (C-term) at 1:1000 dilutionLane 1: A431 whole cell lysatesLane 2: HepG2 whole cell lysatesLane 3: LNCaP whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 31 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit TPI1 Polyclonal Antibody | anti-TPI1 antibody
TPI1 Antibody (C-term)
Gene Names
TPI1; TIM; TPI; TPID; HEL-S-49
Reactivity
HumanPredicted: Bovine, Monkey, Mouse, Rabbit, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
Synonyms
TPI1; Polyclonal Antibody; TPI1 Antibody (C-term); Triosephosphate isomerase; TIM; Triose-phosphate isomerase; TPI; anti-TPI1 antibody
Host
Rabbit
Reactivity
Human
Predicted: Bovine, Monkey, Mouse, Rabbit, Rat
Predicted: Bovine, Monkey, Mouse, Rabbit, Rat
Clonality
Polyclonal
Isotype
Ig
Specificity
This TPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-286 amino acids from the C-terminal region of human TPI1.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Length
249
Applicable Applications for anti-TPI1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
WB: 1:1000
IHC-P: 1:50-100
FC/FACS: 1:10-50
IHC-P: 1:50-100
FC/FACS: 1:10-50
Antigen Source
Human
Antigen Region
258-286
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(All lanes : Anti-TPI1 Antibody (C-term) at 1:1000 dilutionLane 1: A431 whole cell lysatesLane 2: HepG2 whole cell lysatesLane 3: LNCaP whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 31 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-TPI1 Antibody (C-term) at 1:1000 dilutionLane 1: A431 whole cell lysatesLane 2: HepG2 whole cell lysatesLane 3: LNCaP whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 31 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)-Paraffin
(Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with TPI1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)-Paraffin
(Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with TPI1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(TPI1 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(TPI1 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-TPI1 antibody
Description: Purified Rabbit Polyclonal Antibody (Pab)
Background: TPI1 is an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis.
Background: TPI1 is an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis.
References
Martins-de-Souza,D., et.al., BMC Psychiatry 9, 17 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30791
NCBI Official Full Name
triosephosphate isomerase isoform 1
NCBI Official Synonym Full Names
triosephosphate isomerase 1
NCBI Official Symbol
TPI1
NCBI Official Synonym Symbols
TIM; TPI; TPID; HEL-S-49
NCBI Protein Information
triosephosphate isomerase
UniProt Protein Name
Triosephosphate isomerase
UniProt Gene Name
TPI1
UniProt Synonym Gene Names
TPI; TIM
UniProt Entry Name
TPIS_HUMAN
NCBI Description
This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
Research Articles on TPI1
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Product Notes
The TPI1 tpi1 (Catalog #AAA9232008) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TPI1 Antibody (C-term) reacts with Human Predicted: Bovine, Monkey, Mouse, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TPI1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), ELISA (EIA). WB: 1:1000 IHC-P: 1:50-100 FC/FACS: 1:10-50. Researchers should empirically determine the suitability of the TPI1 tpi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TPI1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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