Highly validated and characterized monoclonal/polyclonal
antibodies and recombinant
proteins
The majority of AAA Biotech’s antibodies are highly validated and can be use in multiple
applications such as ELISA, FC,
ICC, IF, IHC, IP, WB, etc. We have antibodies available for rare species, in multiple conjugated
forms or recombinant
antibodies.
As for our high quality proteins, the majority have 90% purity, detected by SDS-PAGE while some are
available in
different tags such as Flag, GST, His, MBP, etc. We also carry high quality native and biologically
active proteins.
AAA Biotech is constantly working to expand our capacity to provide recombinant proteins and
antibodies to most
target proteins.
SELECT `p`.*, `pd`.*, IFNULL(pdns.ncbi_summary, "N/A") as ncbi_summary_pdns, IFNULL(pdns.sp_comments, "N/A") as sp_comments_pdns, IFNULL(pdns.ncbi_research_articles, "N/A") as ncbi_research_articles_pdns, IFNULL(pe.products_description_extra, "N/A") as products_description_extra
FROM (`products`, `products` as `p`)
LEFT OUTER JOIN `products_description` as `pd` ON `p`.`products_id` = `pd`.`products_id`
LEFT OUTER JOIN `products_description_ncbi_sp` as `pdns` ON `p`.`products_id` = `pdns`.`products_id`
LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '15197'
AND `pd`.`language_id` = 1
LIMIT 1
Query
Database
1.88 ms
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
where p.products_id = '15197' and pd.language_id = 1
Query
Database
1.63 ms
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
WHERE `products_attributes`.`products_id` = '15197'
Database (4 total Queries, 4 of them unique across 2 Connections)
Time
Query String
1.95 ms
SELECT `p`.*, `pd`.*, IFNULL(pdns.ncbi_summary, "N/A") as ncbi_summary_pdns, IFNULL(pdns.sp_comments, "N/A") as sp_comments_pdns, IFNULL(pdns.ncbi_research_articles, "N/A") as ncbi_research_articles_pdns, IFNULL(pe.products_description_extra, "N/A") as products_description_extra
FROM (`products`, `products` as `p`)
LEFT OUTER JOIN `products_description` as `pd` ON `p`.`products_id` = `pd`.`products_id`
LEFT OUTER JOIN `products_description_ncbi_sp` as `pdns` ON `p`.`products_id` = `pdns`.`products_id`
LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '15197'
AND `pd`.`language_id` = 1
LIMIT 1
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
where p.products_id = '15197' and pd.language_id = 1
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
WHERE `products_attributes`.`products_id` = '15197'
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This assay has high sensitivity and excellent specificity for detection of rat KGF. No significant cross-reactivity or interference between rat KGF and analogues was observed.
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⇄form => string (3) "N/A"
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⇄concentration => string (3) "N/A"
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⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (727) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
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Activated Point Mutants Of FGFR2 Pathway||1010497!!Adaptive Immune System Pathway||1010925!!Constitutive PI3K/AKT Signaling In Cancer Pathway||1010535!!DAP12 Interactions Pathway||1011029!!DAP12 Signaling Pathway||1011030!!Disease Pathway||1010484!!Downstream Signal Transduction Pathway||1010117!!Downstream Signaling Events Of B Cell Receptor (BCR) Pathway||1010939!!Downstream Signaling Of Activated FGFR Pathway||1010048!!FGFR Ligand Binding And Activation Pathway||1010034
⇄⧉specificity => string (175) "This assay has high sensitivity and excellent specificity for detection of r...
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This assay has high sensitivity and excellent specificity for detection of rat KGF. No significant cross-reactivity or interference between rat KGF and analogues was observed.
⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
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⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (727) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉ncbi_pathways => string (477) "Activated Point Mutants Of FGFR2 Pathway||1010497!!Adaptive Immune System Pa...
$value->a['ncbi_pathways']
Activated Point Mutants Of FGFR2 Pathway||1010497!!Adaptive Immune System Pathway||1010925!!Constitutive PI3K/AKT Signaling In Cancer Pathway||1010535!!DAP12 Interactions Pathway||1011029!!DAP12 Signaling Pathway||1011030!!Disease Pathway||1010484!!Downstream Signal Transduction Pathway||1010117!!Downstream Signaling Events Of B Cell Receptor (BCR) Pathway||1010939!!Downstream Signaling Of Activated FGFR Pathway||1010048!!FGFR Ligand Binding And Activation Pathway||1010034
⇄⧉specificity => string (175) "This assay has high sensitivity and excellent specificity for detection of r...
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This assay has high sensitivity and excellent specificity for detection of rat KGF. No significant cross-reactivity or interference between rat KGF and analogues was observed.
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⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (727) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉ncbi_pathways => string (477) "Activated Point Mutants Of FGFR2 Pathway||1010497!!Adaptive Immune System Pa...
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Activated Point Mutants Of FGFR2 Pathway||1010497!!Adaptive Immune System Pathway||1010925!!Constitutive PI3K/AKT Signaling In Cancer Pathway||1010535!!DAP12 Interactions Pathway||1011029!!DAP12 Signaling Pathway||1011030!!Disease Pathway||1010484!!Downstream Signal Transduction Pathway||1010117!!Downstream Signaling Events Of B Cell Receptor (BCR) Pathway||1010939!!Downstream Signaling Of Activated FGFR Pathway||1010048!!FGFR Ligand Binding And Activation Pathway||1010034
⇄⧉specificity => string (373) "This assay has high sensitivity and excellent specificity for detection of A...
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This assay has high sensitivity and excellent specificity for detection of ATP. No significant cross-reactivity or interference between ATP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATP and all the analogues, therefore, cross reaction may still exist in some cases.
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⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1374) "Principle of the Assay: ATP ELISA kit applies the competitive enzyme immunoa...
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Principle of the Assay: ATP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ATP antibody and an ATP-HRP conjugate. The assay sample and buffer are incubated together with ATP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP concentration since ATP from samples and ATP-HRP conjugate compete for the anti-ATP antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP from the sample, fewer sites are left to bind ATP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This ATP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ATP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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⇄ncbi_pathways => string (3) "N/A"
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⇄sp_protein_name_syn => string (3) "N/A"
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⇄⧉search_terms => string (527) "aaa16131 rat this assay has high sensitivity and excellent specificity for d...
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aaa16131 rat this assay has high sensitivity and excellent specificity for detection of atp no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa16131_sc elisa kit adenosine triphosphate samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 1.0 ng ml competitive1.0
Assay Type||Quantitative Sandwich!!Samples||Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids!!Detection Range||20-6000nmol/L!!Sensitivity||10.43nmol/L
⇄⧉etc_term2 => string (403) "Intra-assay Precision||Intra-Assay Precision (Precision within an assay) Thr...
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Intra-assay Precision||Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%!!Inter-assay Precision||Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
⇄⧉products_description => string (882) "Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (EL...
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Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human ATP antibody. ATP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ATP Antibody is added and binds to ATP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ATP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ATP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.<br><br>Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Adenosine triphosphate (also known as ATP) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
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⇄⧉search_terms => string (302) "aaa11324 human typical testing data standard curve for reference only aaa113...
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aaa11324 human typical testing data standard curve for reference only aaa11324_sc elisa kit adenosine triphosphate atp samples serum plasma cell culture supernates lysates tissue homogenates assay type quantitative sandwich detection range 20nmol l 6000nmol sensitivity 10.43nmol intra cv<8 inter cv<10
Assay Type||Quantitative Sandwich!!Samples||Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids!!Detection Range||5-1500ng/ml!!Sensitivity||2.57ng/ml
⇄⧉etc_term2 => string (403) "Intra-assay Precision||Intra-Assay Precision (Precision within an assay) Thr...
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Intra-assay Precision||Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%!!Inter-assay Precision||Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
⇄⧉products_description => string (874) "Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (EL...
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Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat ATP antibody. ATP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ATP Antibody is added and binds to ATP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ATP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat ATP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.<br><br>Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Adenosine triphosphate (also known as ATP) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
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⇄⧉search_terms => string (460) "aaa11325 rat typical testing data standard curve for reference only aaa11325...
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aaa11325 rat typical testing data standard curve for reference only aaa11325_sc elisa kit adenosine triphosphate atp samples serum plasma cell culture supernates ascites tissue homogenates or other biological fluids assay type quantitative sandwich detection range 5 1500ng ml sensitivity 2.57ng intra precision within an three of known concentration were tested on one plate to assess cv<8 inter between assays in separate cv = sd mean x 100 cv<10 range5 x100
⇄⧉products_description => string (824) "Principle of the Assay: As mentioned above, this kit utilizes the Double Ant...
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Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human AD monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
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aaa22688 human no cross reaction with other factors typical testing data standard curve for reference only aaa22688_sc elisa kit adenosine ad samples serum plasma or cell culture supernatant detection range 1000 ng ml 15.6 sensitivity 5 intra assay precision ? 8 inter 12 sensitivity5 ?8 inter12
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Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human ADO monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
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Intended Uses: This ADA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADA. This ELISA kit for research use only!<br><br>Principle of the Assay: ADA ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ADA antibody and an ADA-HRP conjugate. The assay sample and buffer are incubated together with ADA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADA concentration since ADA from samples and ADA-HRP conjugate compete for the anti-ADA antibody binding site. Since the number of sites is limited, as more sites are occupied by ADA from the sample, fewer sites are left to bind ADA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADA concentration in each sample is interpolated from this standard curve.
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aaa16650 human typical testing data standard curve for reference only aaa16650_td elisa kit adenosine deaminase ada aminohydrolase 40,764 da ada1 ada_human 47078295 np_000013.2 p00813 nm_000022.2 q53f92 q6la59 608958 samples serum plasma cell culture supernatants body fluid and tissue homogenate assay type quantitative competitive sensitivity 0.1 ng ml sensitivity0.1
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Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse ADO monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
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aaa22664 mouse no cross reaction with other factors typical testing data standard curve for reference only aaa22664_sc elisa kit adenosine ado samples serum plasma or cell culture supernatant detection range 100 ng ml 1.56 sensitivity 0.5 intra assay precision ? 8 inter 12 range100 sensitivity0.5 ?8 inter12
⇄⧉specificity => string (376) "This assay has high sensitivity and excellent specificity for detection of I...
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This assay has high sensitivity and excellent specificity for detection of IP-3. No significant cross-reactivity or interference between IP-3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IP-3 and all the analogues, therefore, cross reaction may still exist in some cases.
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⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1389) "Principle of the Assay: IP-3 ELISA kit applies the competitive enzyme immuno...
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Principle of the Assay: IP-3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IP-3 antibody and an IP-3-HRP conjugate. The assay sample and buffer are incubated together with IP-3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IP-3 concentration since IP-3 from samples and IP-3-HRP conjugate compete for the anti-IP-3 antibody binding site. Since the number of sites is limited, as more sites are occupied by IP-3 from the sample, fewer sites are left to bind IP-3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IP-3 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This IP-3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse IP-3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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aaa17090 mouse this assay has high sensitivity and excellent specificity for detection of ip 3 no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa17090_sc elisa kit inositol triphosphate ip3 samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 0.1 ng ml competitive0.1
⇄⧉products_description => string (863) "Intended Uses: This cADPR-competitive ELISA kit is intended for Laboratory R...
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Intended Uses: This cADPR-competitive ELISA kit is intended for Laboratory Research use only and is not for use in diagnostic or therapeutic procedures. Add sample and cADPR standards in the plate wells where cADPR antibodies have been coated. Then add HRPlabeled mouse cADPR antigen. The unreacted component will be removed after incubating and washing, and the solid-phase antibody-enzyme-labeled antigen immune complex on the solid surface of the microplate will remain. After adding Chromogen Solution A and Chromogen Solution B, the immune complex will change to blue by HRP catalyzing. It will change to yellow at the end when the adding stop solution works. Measure the absorbance (OD value) at 450 nm using a microtiter plate reader. The concentration of cADPR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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aaa23737 mouse typical testing data standard curve for reference only aaa23737_sc elisa kit cyclic adenosine diphosphate ribose
⇄⧉specificity => string (167) "This assay has high sensitivity and excellent specificity for detection of U...
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This assay has high sensitivity and excellent specificity for detection of UTP. No significant cross-reactivity or interference between UTP and analogues was observed.
⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
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⇄concentration => string (3) "N/A"
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. <br>To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay Type||Quantitative Competitive!!Samples||Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids!!Detection Range||61.7-5,000ng/mL!!Sensitivity||< 28.5ng/mL
⇄⧉etc_term2 => string (412) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 ...
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level UTP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%!!Inter-assay Precision||Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level UTP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
⇄⧉products_description => string (978) "Principle of the Assay: This assay employs the competitive inhibition enzyme...
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Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to UTP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled UTP and unlabeled UTP (Standards or samples) with the pre-coated antibody specific to UTP. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of UTP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of UTP in the sample.<br><br>Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of UTP in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
<a href="https://www.sciencedirect.com/science/article/pii/S0925443918303958" target="_blank">Epigenetic upregulation and functional role of the mitochondrial aspartate/glutamate carrier isoform 1 in hepatocellular carcinoma; Biochim Biophys Acta Mol Basis Dis; 2019 Jan;1865(1):38-47.; PMID: 30321589</a>; Vittoria Infantino; Department of Science, University of Basilicata, viale dell"Ateneo Lucano 10, 85100 Potenza, Italy; Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, via Orabona 4, 70125 Bari, Italy. Electronic address: [email protected].
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⇄⧉search_terms => string (623) "aaa20805 general this assay has high sensitivity and excellent specificity f...
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aaa20805 general this assay has high sensitivity and excellent specificity for detection of utp no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa20805_sc elisa kit uridine triphosphate samples serum plasma tissue homogenates cell lysates culture supernates other biological fluids type quantitative competitive range 61.7 5,000ng ml < 28.5ng intra precision within an 3 with low middle level were tested 20 times on one plate respectively cv<10 inter assays different plates 8 replicates in each cv = sd meanx100 cv<12 an3 tested20 plates8
⇄specificity => string (74) "Specifically recognize IP3, no obvious cross reaction with other analogues"
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⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
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⇄concentration => string (3) "N/A"
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⇄⧉storage_stability => string (156) "Store entire kit at 2-8C for short-term. For longer-term, please store the m...
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Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Assay Type||Competitive!!Samples||Serum, plasma, cell culture supernatant and other biological samples!!Detection Range||1.563-100pg/ml!!Sensitivity||0.938pg/ml
⇄⧉etc_term2 => string (270) "Intra-assay Precision||Intra-assay Precision: samples with low, medium and h...
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Intra-assay Precision||Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.!!Inter-assay Precision||Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
⇄⧉products_description => string (1473) "Background: Inositol-1,4,5-trisphosphate [Ins(1,4,5)P3] is a second messenge...
$value[10]['_source']['products_description']
Background: Inositol-1,4,5-trisphosphate [Ins(1,4,5)P3] is a second messenger for many growth factors, hormones, and neurotransmitters. It is produced in cells by phospholipase C (PLC)-mediated hydrolysis of phosphatidyl inositol- 4,5-bisphosphate. Ins(1,4,5)P3 binds to one of several inositol-1,4,5-trisphosphate receptors (IP3 receptors), each containing a calcium channel domain. Binding of Ins(1,4,5)P3 to the receptor releases calcium from intracellular stores, leading to a dramatic, but transient, increase in the cytosolic concentration of free calcium<br><br>Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with IP3. During the reaction, IP3 in the sample or standard competes with a fixed amount of IP3 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to IP3. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of IP3 in the samples is then determined by comparing the OD of the samples to the standard curve. The concentration of the target substance was inversely proportional to the OD450 value.
⇄⧉search_terms => string (592) "aaa27496 general this assay has high sensitivity and excellent specificity f...
$value[10]['_source']['search_terms']
aaa27496 general this assay has high sensitivity and excellent specificity for detection of hsd3b1 no significant cross reactivity or interference between analogues is observed typical testing data standard curve reference only aaa27496_sc elisa kit ip3 inositol triphosphate universal samples serum plasma tissue homogenates other biological fluids type competitive range 0.312 20ng ml <0.188ng intra precision within an 3 with low middle level were tested 20 times on one plate respectively cv<8 inter assays different plates 8 replicates in each cv = sd meanx100 cv<10 an3 tested20 plates8
⇄⧉products_description => string (674) "Principle of the Assay: This experiment use double-sandwich elisa technique ...
$value[11]['_source']['products_description']
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human ADA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
⇄products_references => string (3) "N/A"
$value[11]['_source']['products_references']
⇄⧉products_related_diseases => string (198) "Neoplasms||575!!Nervous System Diseases||412!!Brain Diseases||202!!Heart Dis...
⇄⧉search_terms => string (415) "aaa12540 human no cross reaction with other factors typical testing data sta...
$value[11]['_source']['search_terms']
aaa12540 human no cross reaction with other factors typical testing data standard curve for reference only aaa12540_sc elisa kit adenosine deaminase ada aminohydrolase 40,764 da ada1 ada_human 47078295 np_000013.2 p00813 nm_000022.2 q53f92 q6la59 608958 samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 100 ng ml 1.56 sensitivity up to 0.5ng intra precision range100
⇄⧉specificity => string (185) "This assay has high sensitivity and excellent specificity for detection of h...
$value[12]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of human ENTPD1. No significant cross-reactivity or interference between human ENTPD1 and analogues was observed.
⇄purity => string (3) "N/A"
$value[12]['_source']['purity']
⇄form => string (3) "N/A"
$value[12]['_source']['form']
⇄concentration => string (3) "N/A"
$value[12]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[12]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
$value[12]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (739) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
$value[12]['_source']['products_description']
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ENTPD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ENTPD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ENTPD1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ENTPD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (770) "aaa18071 human this assay has high sensitivity and excellent specificity for...
$value[12]['_source']['search_terms']
aaa18071 human this assay has high sensitivity and excellent specificity for detection of entpd1 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa18071_td elisa kit ectonucleoside triphosphate diphosphohydrolase 1 atpdase cd39 dkfzp686d194 dkfzp686i093 flj40921 flj40959 ntpdase antigen ecto atp apyrase lymphoid cell activation isoform 2 spg64 atpase 57,965 da cd_antigen entp1_human 147905700 np_001091645.1 p49961 nm_001098175.1 q5t561 q5t562 q86vv3 q9uqq9 q9y3q9 a9z1x8 b4dwb9 b4e1x1 b7z599 g3xaf6 gene 615683 samples serum plasma tissue homogenates type quantitative sandwich range 23.44 pg ml 1500 typically less than 5.86 intra precision within an cv diphosphohydrolase1 isoform2
⇄⧉specificity => string (167) "This assay has high sensitivity and excellent specificity for detection of I...
$value[13]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of IP3. No significant cross-reactivity or interference between IP3 and analogues was observed.
⇄purity => string (3) "N/A"
$value[13]['_source']['purity']
⇄form => string (3) "N/A"
$value[13]['_source']['form']
⇄concentration => string (3) "N/A"
$value[13]['_source']['concentration']
⇄⧉storage_stability => string (475) "The stability of kit is determined by the loss rate of activity. The loss ra...
$value[13]['_source']['storage_stability']
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. <br>To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay Type||Competitive!!Samples||Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids!!Detection Range||123.5-10,000pg/mL!!Sensitivity||45.4pg/mL
⇄⧉etc_term2 => string (412) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 ...
$value[13]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IP3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%!!Inter-assay Precision||Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IP3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
⇄products_name_syn => string (41) "ELISA Kit for Inositol Triphosphate (IP3)"
$value[13]['_source']['products_name_syn']
⇄products_gene_name => string (3) "IP3"
$value[13]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[13]['_source']['products_gene_name_syn']
⇄⧉products_description => string (978) "Intended Uses: The kit is a competitive inhibition enzyme immunoassay techni...
$value[13]['_source']['products_description']
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of IP3 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.<br><br>Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to IP3 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled IP3 and unlabeled IP3 (Standards or samples) with the pre-coated antibody specific to IP3. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of IP3 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of IP3 in the sample.
Signal transduction; Metabolic pathway; Endocrinology; Cardiovascular biology; Neuroscience; Hormone metabolism; Bone metabolism
⇄ncbi_full_name => string (3) "N/A"
$value[13]['_source']['ncbi_full_name']
⇄ncbi_full_name_syn => string (3) "N/A"
$value[13]['_source']['ncbi_full_name_syn']
⇄ncbi_symbol => string (3) "N/A"
$value[13]['_source']['ncbi_symbol']
⇄ncbi_symbol_syn => string (3) "N/A"
$value[13]['_source']['ncbi_symbol_syn']
⇄ncbi_protein_info => string (3) "N/A"
$value[13]['_source']['ncbi_protein_info']
⇄ncbi_chrom_loc => string (3) "N/A"
$value[13]['_source']['ncbi_chrom_loc']
⇄ncbi_gene_id => string (3) "N/A"
$value[13]['_source']['ncbi_gene_id']
⇄ncbi_mol_weight => string (3) "N/A"
$value[13]['_source']['ncbi_mol_weight']
⇄ncbi_pathways => string (3) "N/A"
$value[13]['_source']['ncbi_pathways']
⇄sp_protein_name => string (3) "N/A"
$value[13]['_source']['sp_protein_name']
⇄sp_protein_name_syn => string (3) "N/A"
$value[13]['_source']['sp_protein_name_syn']
⇄sp_gene_name => string (3) "N/A"
$value[13]['_source']['sp_gene_name']
⇄sp_gene_name_syn => string (3) "N/A"
$value[13]['_source']['sp_gene_name_syn']
⇄sp_entry_name => string (3) "N/A"
$value[13]['_source']['sp_entry_name']
⇄sp_mim => string (3) "N/A"
$value[13]['_source']['sp_mim']
⇄sp_interactions => string (3) "N/A"
$value[13]['_source']['sp_interactions']
⇄products_url => string (3) "N/A"
$value[13]['_source']['products_url']
⇄products_viewed => string (1) "0"
$value[13]['_source']['products_viewed']
⇄⧉search_terms => string (743) "aaa20058 most species this assay has high sensitivity and excellent specific...
$value[13]['_source']['search_terms']
aaa20058 most species this assay has high sensitivity and excellent specificity for detection of ip3 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa20058_sc elisa kit inositol triphosphate signal transduction metabolic pathway endocrinology cardiovascular biology neuroscience hormone metabolism bone samples serum plasma tissue homogenates cell lysates culture supernates other biological fluids type quantitative competitive range 123.5 10,000pg ml < 50.1pg intra precision within an 3 with low middle level were tested 20 times on one plate respectively cv<10 inter assays different plates 8 replicates in each cv = sd meanx100 cv<12 an3 tested20 plates8
⇄⧉specificity => string (373) "This assay has high sensitivity and excellent specificity for detection of A...
$value[14]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of ADP. No significant cross-reactivity or interference between ADP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADP and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[14]['_source']['purity']
⇄form => string (3) "N/A"
$value[14]['_source']['form']
⇄concentration => string (3) "N/A"
$value[14]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1328) "Intended Uses: This ADP ELISA kit is a 1.5 hour solid-phase ELISA designed f...
$value[14]['_source']['products_description']
Intended Uses: This ADP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADP. This ELISA kit for research use only!<br><br>Principle of the Assay: ADP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ADP antibody and an ADP-HRP conjugate. The assay sample and buffer are incubated together with ADP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADP concentration since ADP from samples and ADP-HRP conjugate compete for the anti-ADP antibody binding site. Since the number of sites is limited, as more sites are occupied by ADP from the sample, fewer sites are left to bind ADP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADP concentration in each sample is interpolated from this standard curve.
⇄⧉search_terms => string (548) "aaa17161 human this assay has high sensitivity and excellent specificity for...
$value[14]['_source']['search_terms']
aaa17161 human this assay has high sensitivity and excellent specificity for detection of adp no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa17161_sc elisa kit adenosine diphosphate signal transduction samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 1.0 ng ml competitive1.0
⇄⧉products_description => string (1471) "<b>Intended Uses: </b>This AMAP ELISA kit is a 1.5 hour solid-phase ELISA de...
$value[15]['_source']['products_description']
<b>Intended Uses: </b>This AMAP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken AMAP. This ELISA kit for research use only!<br><br><b>Principle of the Assay: </b>AMAP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AMAP. Standards or samples are then added to the microtiter plate wells and AMAP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AMAP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AMAP are added to each well to "sandwich" the AMAP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AMAP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMAP concentration in each sample is interpolated from this standard curve.
⇄⧉search_terms => string (284) "aaa16995 chicken typical testing data standard curve for reference only aaa1...
$value[15]['_source']['search_terms']
aaa16995 chicken typical testing data standard curve for reference only aaa16995_td elisa kit adenosine monophosphate activated protein ampk cancer samples serum plasma cell culture supernatants body fluid and tissue homogenate assay type sandwich sensitivity 0.1 ng ml sensitivity0.1
Assay Type||Quantitative Sandwich!!Samples||Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids!!Detection Range||0.5-200U/L!!Sensitivity||0.25U/L
⇄⧉etc_term2 => string (403) "Intra-assay Precision||Intra-Assay Precision (Precision within an assay) Thr...
$value[16]['_source']['etc_term2']
Intra-assay Precision||Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%!!Inter-assay Precision||Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Rat Phosphorylated adenosine monophosphate activated protein kinase, AMPK ELISA Kit
⇄products_name_syn => string (3) "N/A"
$value[16]['_source']['products_name_syn']
⇄products_gene_name => string (4) "AMPK"
$value[16]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[16]['_source']['products_gene_name_syn']
⇄⧉products_description => string (922) "Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (EL...
$value[16]['_source']['products_description']
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat AMPK antibody. AMPK present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat AMPK Antibody is added and binds to AMPK in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated AMPK antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat AMPK. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.<br><br>Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Phosphorylated adenosine monophosphate activated protein kinase (also known as AMPK) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
⇄⧉search_terms => string (467) "aaa11255 rat typical testing data standard curve for reference only aaa11255...
$value[16]['_source']['search_terms']
aaa11255 rat typical testing data standard curve for reference only aaa11255_sc elisa kit phosphorylated adenosine monophosphate activated protein kinase ampk samples serum plasma cell culture supernates lysates tissue homogenates assay type quantitative sandwich detection range 0.5u l 200u sensitivity 0.25u intra precision within an three of known concentration were tested on one plate to assess cv<8 inter between assays in separate cv = sd mean x 100 cv<10 x100
⇄⧉specificity => string (382) "This assay has high sensitivity and excellent specificity for detection of E...
$value[17]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of ENTPD1. No significant cross-reactivity or interference between ENTPD1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ENTPD1 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[17]['_source']['purity']
⇄form => string (3) "N/A"
$value[17]['_source']['form']
⇄concentration => string (3) "N/A"
$value[17]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1416) "Principle of the Assay: ENTPD1 ELISA kit applies the competitive enzyme immu...
$value[17]['_source']['products_description']
Principle of the Assay: ENTPD1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ENTPD1 antibody and an ENTPD1-HRP conjugate. The assay sample and buffer are incubated together with ENTPD1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ENTPD1 concentration since ENTPD1 from samples and ENTPD1-HRP conjugate compete for the anti-ENTPD1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ENTPD1 from the sample, fewer sites are left to bind ENTPD1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ENTPD1 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This ENTPD1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey ENTPD1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (734) "aaa27262 monkey this assay has high sensitivity and excellent specificity fo...
$value[17]['_source']['search_terms']
aaa27262 monkey this assay has high sensitivity and excellent specificity for detection of entpd1 no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa27262_sc elisa kit ectonucleoside triphosphate diphosphohydrolase 1 ntpdase atpdase ecto apyrase atpase atp lymphoid cell activation antigen 57,408 da cd_antigen cd39 entp1_rat 12018242 np_072109.1 p97687 nm_022587.1 immunology samples serum plasma culture supernatants body fluid tissue homogenate type quantitative competitive 1.0 pg ml diphosphohydrolase1 competitive1.0
⇄⧉specificity => string (167) "This assay has high sensitivity and excellent specificity for detection of A...
$value[18]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of ADA. No significant cross-reactivity or interference between ADA and analogues was observed.
⇄purity => string (3) "N/A"
$value[18]['_source']['purity']
⇄form => string (3) "N/A"
$value[18]['_source']['form']
⇄concentration => string (3) "N/A"
$value[18]['_source']['concentration']
⇄⧉storage_stability => string (475) "The stability of kit is determined by the loss rate of activity. The loss ra...
$value[18]['_source']['storage_stability']
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. <br>To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay Type||Double-antibody Sandwich!!Samples||Serum, Plasma, Tissue homogenates and other biological fluids!!Detection Range||0.156-10ng/mL!!Sensitivity||0.061ng/mL
⇄⧉etc_term2 => string (412) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 ...
$value[18]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ADA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%!!Inter-assay Precision||Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ADA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
⇄⧉products_description => string (979) "Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantit...
$value[18]['_source']['products_description']
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ADA in human serum, plasma, tissue homogenates and other biological fluids.<br><br>Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ADA. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ADA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ADA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
⇄products_references => string (3) "N/A"
$value[18]['_source']['products_references']
⇄⧉products_related_diseases => string (204) "Nervous System Diseases||459!!Brain Diseases||225!!Necrosis||212!!Inflammati...
⇄⧉search_terms => string (639) "aaa20439 human this assay has high sensitivity and excellent specificity for...
$value[18]['_source']['search_terms']
aaa20439 human this assay has high sensitivity and excellent specificity for detection of ada no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa20439_sc elisa kit adenosine deaminase aminohydrolase 40,764 da ada1 ada_human 47078295 np_000013.2 p00813 nm_000022.2 q53f92 q6la59 102700 enzyme kinase tumor immunity infection samples serum plasma tissue homogenates other biological fluids type quantitative sandwich range 0.156 10ng ml < 0.061ng intra precision within an 3 with low middle level were tested 20 times on one plate respectively cv an3 tested20
Bone Marrow||207!!Liver||185!!Lung||185!!Thymus||130!!Skin||113!!Intestine||80!!Eye||75!!Connective Tissue||50!!Embryonic Tissue||36!!Pancreas||24
⇄⧉ncbi_gi_num => string (9) "440309866"
$value[19]['_source']['ncbi_gi_num']
1983-12-15 04:17:46 UTC
⇄ncbi_acc_num => string (14) "NP_001258981.1"
$value[19]['_source']['ncbi_acc_num']
⇄ncbi_acc_num_related => string (3) "N/A"
$value[19]['_source']['ncbi_acc_num_related']
⇄ncbi_sp_acc_num_related => string (6) "P03958"
$value[19]['_source']['ncbi_sp_acc_num_related']
⇄ncbi_gb_acc_num => string (14) "NM_001272052.1"
$value[19]['_source']['ncbi_gb_acc_num']
⇄sp_acc_num => string (6) "P03958"
$value[19]['_source']['sp_acc_num']
⇄sp_acc_num_syn => string (3) "N/A"
$value[19]['_source']['sp_acc_num_syn']
⇄products_image => string (8) "Antibody"
$value[19]['_source']['products_image']
⇄sequence_positions => string (3) "N/A"
$value[19]['_source']['sequence_positions']
⇄sequence_length => string (3) "352"
$value[19]['_source']['sequence_length']
⇄sequence => string (3) "N/A"
$value[19]['_source']['sequence']
⇄clonality => string (10) "Polyclonal"
$value[19]['_source']['clonality']
⇄isotype => string (3) "N/A"
$value[19]['_source']['isotype']
⇄clone_number => string (3) "N/A"
$value[19]['_source']['clone_number']
⇄host => string (6) "Rabbit"
$value[19]['_source']['host']
⇄reactivity => string (54) "Mouse, Rat<br>No cross reactivity with other proteins."
$value[19]['_source']['reactivity']
⇄specificity => string (3) "N/A"
$value[19]['_source']['specificity']
⇄purity => string (3) "N/A"
$value[19]['_source']['purity']
⇄form => string (11) "Lyophilized"
$value[19]['_source']['form']
⇄concentration => string (3) "N/A"
$value[19]['_source']['concentration']
⇄⧉storage_stability => string (202) "Store at -20 degree C for one year. After reconstitution, at 4 degree C for ...
$value[19]['_source']['storage_stability']
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
⇄app_tested => string (58) "ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)"
⇄⧉testing_protocols => string (4478) "IHC (Immunohistchemistry)||Figure 6. IHC analysis of ADA using anti-ADA anti...
$value[19]['_source']['testing_protocols']
IHC (Immunohistchemistry)||Figure 6. IHC analysis of ADA using anti-ADA antibody (AAA19140).<br>ADA was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ADA Antibody (AAA19140) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.<br>||AAA19140_IHC6.jpg!!IHC (Immunohistochemistry)||Figure 5. IHC analysis of ADA using anti-ADA antibody (AAA19140).<br>ADA was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ADA Antibody (AAA19140) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.<br>||AAA19140_IHC5.jpg!!IHC (Immunohistochemistry)||Figure 4. IHC analysis of ADA using anti-ADA antibody (AAA19140).<br>ADA was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ADA Antibody (AAA19140) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.<br>||AAA19140_IHC4.jpg!!IHC (Immunohistochemistry)||Figure 3. IHC analysis of ADA using anti-ADA antibody (AAA19140).ADA was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ADA Antibody (AAA19140) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.||AAA19140_IHC3.jpg!!IHC (Immunohistochemistry)||Figure 2. IHC analysis of ADA using anti-ADA antibody (AAA19140).ADA was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ADA Antibody (AAA19140) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.||AAA19140_IHC2.jpg!!WB (Western Blot)||Figure 1. Western blot analysis of ADA using anti-ADA antibody (AAA19140).<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>Lane 1: rat stomach tissue lysates,<br>Lane 2: rat kidney tissue lysates,<br>Lane 3: mouse small intestine tissue lysates,<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADA antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADA at approximately 45KD. The expected band size for ADA is at 40KD.||AAA19140_WB.jpg
⇄⧉etc_term1 => string (482) "Immunogen||E Coli-derived mouse ADA recombinant protein (Position: A2-H238)....
$value[19]['_source']['etc_term1']
Immunogen||E Coli-derived mouse ADA recombinant protein (Position: A2-H238).!!Subcellular Localization||Cell membrane; Peripheral membrane protein; Extracellular side. Cell junction.!!Tissue Specificity||Detected in brain neurons in the median emninence (at protein level) (PubMed: 8783262). Expressed in secondary deciduum (at protein level) (PubMed: 9272950). Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues.
⇄⧉etc_term2 => string (169) "Reconstitution||Add 0.2ml of distilled water will yield a concentration of 5...
$value[19]['_source']['etc_term2']
Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.!!Contents||Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
⇄⧉products_description => string (2075) "Description: Adenosine Deaminase (also known as adenosine aminohydrolase, or...
$value[19]['_source']['products_description']
Description: Adenosine Deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. Primarily, ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.<br>Protein Function: Catalyzes the hydrolytic deamination of adenosine and 2- deoxyadenosine (PubMed: 9272950). Plays an important role in purine metabolism and in adenosine homeostasis (PubMed: 9272950, PubMed: 10720488). Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events (PubMed: 11435465). Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion (By similarity). Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (By similarity). Enhances CD4+ T-cell differentiation and proliferation (By similarity). Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change (By similarity). Stimulates plasminogen activation (By similarity). Plays a role in male fertility (By similarity). Plays a protective role in early postimplantation embryonic development (PubMed: 9272950).
⇄products_references => string (3) "N/A"
$value[19]['_source']['products_references']
⇄⧉products_related_diseases => string (213) "Neoplasms||551!!Cardiovascular Diseases||438!!Nervous System Diseases||388!!...
Epigenetics and Nuclear Signaling; Chromatin Modifying Enzymes; deamination; Cancer; Cancer Metabolism; Response to Hypoxia; Metabolism; Pathways and Processes; Metabolism Processes
⇄⧉search_terms => string (1845) "aaa19140 rabbit mouse rat no cross reactivity with other proteins polyclonal...
$value[19]['_source']['search_terms']
aaa19140 rabbit mouse rat no cross reactivity with other proteins polyclonal lyophilized elisa eia immunohistochemistry ihc western blot wb 0.1 0.5mug ml paraffin embedded section 0.5 1mug direct figure 1 analysis of ada using anti antibody electrophoresis was performed on a 5 20 sds page gel at 70v stacking 90v resolving for 2 3 hours the sample well each lane loaded 50ug under reducing conditions stomach tissue lysates kidney small intestine after were transferred to nitrocellulose membrane 150ma 50 90 minutes blocked non fat milk tbs 1.5 hour rt incubated antigen affinity purified 0.5ug overnight 4 degree c then washed tween times and probed goat igg hrp secondary dilution 1:10000 signal is developed an enhanced chemiluminescent detection ecl kit tanon 5200 system specific band detected approximately 45kd expected size 40kd aaa19140_wb in heat mediated retrieval citrate buffer ph6 epitope solution mins 10 serum 1ug biotinylated used as 30 37 strepavidin biotin complex sabc dab chromogen aaa19140_ihc2 spleen aaa19140_ihc3 aaa19140_ihc4 brain aaa19140_ihc5 6 aaa19140_ihc6 adenosine deaminase picoband 39,992 da aminohydrolase 440309866 np_001258981.1 p03958 nm_001272052.1 epigenetics nuclear signaling chromatin modifying enzymes deamination cancer metabolism response hypoxia pathways processes immunogen e coli derived recombinant protein position a2 h238 subcellular localization cell peripheral extracellular side junction specificity neurons median emninence level pubmed 8783262 expressed deciduum 9272950 found all tissues occurs large amounts t lymphocytes time weaning gastrointestinal reconstitution add 0.2ml distilled water will yield concentration 500ug contents vial contains 4mg trehalose 0.9mg nacl 0.2mg na2hpo4 0.05mg nan3 wb0.1 section0.5 figure1 a5 for2 150ma50 tbs1.5 overnight4 mins10 as30 aaa19140_ihc56
