Flow Cytometry (FC/FACS)
(Overlay histogram showing U-2OS cells stained at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Rabbit anti-Human CF150 Polyclonal Antibody | anti-CF150 antibody
CF150 Antibody (Center)
WB: 1:1000
Flow Cytometry (FC/FACS)
(Overlay histogram showing U-2OS cells stained at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Flow Cytometry (FC/FACS)
(Overlay histogram showing U-2OS cells stained at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Western Blot (WB)
(All lanes : Anti-CF150 Antibody (Center) at 1:1000 dilutionLane 1: human cerebellum lysatesLane 2: human spleen lysatesLane 3: HT-29 whole cell lysatesLane 4: MCF-7 whole cell lysatesLane 5: THP-1 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 59 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-CF150 Antibody (Center) at 1:1000 dilutionLane 1: human cerebellum lysatesLane 2: human spleen lysatesLane 3: HT-29 whole cell lysatesLane 4: MCF-7 whole cell lysatesLane 5: THP-1 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 59 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Background: The exact function of C6orf150 remains unknown.
NCBI and Uniprot Product Information
Uniprot Description
MB21D1: Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.
Protein type: Enzyme, cellular metabolism; EC 2.7.7.86
Chromosomal Location of Human Ortholog: 6q13
Cellular Component: cytosol
Molecular Function: ATP binding; DNA binding; GTP binding; metal ion binding
Biological Process: activation of innate immune response; cyclic nucleotide biosynthetic process; defense response to virus; innate immune response; positive regulation of defense response to virus by host; positive regulation of interferon type I production
Research Articles on CF150
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Product Notes
The CF150 mb21d1 (Catalog #AAA9231966) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CF150 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CF150 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). FC/FACS: 1:25 WB: 1:1000. Researchers should empirically determine the suitability of the CF150 mb21d1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CF150, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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