ADAM15 blocking peptide

ADAM15 Blocking Peptide (N-Term)

• Gene Names: ADAM15; MDC15
• Synonyms: ADAM15; ADAM15 Blocking Peptide (N-Term); Disintegrin and metalloproteinase domain-containing protein 15; ADAM 15; 3424-; Metalloprotease RGD disintegrin protein; Metalloproteinase-like; disintegrin-like; and cysteine-rich protein 15; MDC-15; Metargidin; MDC15; ADAM15 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 37-52 of HUMAN ADAM15
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 863

• Cellular Location: Endomembrane system; Single-pass type I membrane protein. Cell junction, adherens junction. Cell projection, cilium, flagellum. Cytoplasmic vesicle, secretory vesicle, acrosome. Note: The majority of the protein is localized in a perinuclear compartment which may correspond to the trans-Golgi network or the late endosome. The pro-protein is the major detectable form on the cell surface, whereas the majority of the protein in the cell is processed (By similarity).
• Tissue Location: Expressed in colon and small intestine. Expressed in airway smooth muscle and glomerular mesangial cells (at protein level). Ubiquitously expressed. Overexpressed in atherosclerotic lesions. Constitutively expressed in cultured endothelium and smooth muscle. Expressed in chondrocytes Expressed in airway smooth muscle and glomerular mesangial cells
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ADAM15 blocking peptide

Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain.

NCBI and Uniprot Product Information

• NCBI GI #: 300669614
• NCBI GeneID: 8751
• UniProt Accession #: Q13444; Q13493; Q53XQ0; Q5SR68; Q5SR69; Q6R267; Q71S61; Q71S62; B3KQU5; B4DLB5; B4DMH8; E9PN65
• Molecular Weight: 68,256 Da
• NCBI Official Full Name: Disintegrin and metalloproteinase domain-containing protein 15
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 15
• NCBI Official Symbol: ADAM15
• NCBI Official Synonym Symbols: MDC15
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 15
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 15
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM15
• UniProt Synonym Gene Names: MDC15; ADAM 15; MDC-15
• UniProt Entry Name: ADA15_HUMAN

NCBI Description

The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAM15: Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; EC 3.4.24.-; Protease; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cell surface; plasma membrane

Molecular Function: integrin binding; metalloendopeptidase activity; metallopeptidase activity; protein binding; SH3 domain binding

Biological Process: apoptosis; cell-matrix adhesion; extracellular matrix disassembly; innate immune response; integrin-mediated signaling pathway; negative regulation of cell growth; negative regulation of cell migration; negative regulation of cell-matrix adhesion