EPHA4 blocking peptide

EPHA4 Blocking Peptide (Center)

• Gene Names: EPHA4; SEK; HEK8; TYRO1
• Synonyms: EPHA4; EPHA4 Blocking Peptide (Center); Ephrin type-A receptor 4; EPH-like kinase 8; EK8; hEK8; Tyrosine-protein kinase TYRO1; Tyrosine-protein kinase receptor SEK; HEK8; SEK; TYRO1; EPHA4 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 383-397 of HUMAN EPHA4
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 986

• Cellular Location: Cell membrane; Single-pass type I membrane protein. Cell projection, axon. Cell projection, dendrite. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Early endosome Note: Clustered upon activation and targeted to early endosome
• Tissue Location: Ubiquitous.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for EPHA4 blocking peptide

Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium.

NCBI and Uniprot Product Information

• NCBI GI #: 1711371
• NCBI GeneID: 2043
• UniProt Accession #: P54764; Q2M380; A8K2P1; B2R601; B7Z6Q8
• Molecular Weight: 104,507 Da
• NCBI Official Full Name: Ephrin type-A receptor 4
• NCBI Official Synonym Full Names: EPH receptor A4
• NCBI Official Symbol: EPHA4
• NCBI Official Synonym Symbols: SEK; HEK8; TYRO1
• NCBI Protein Information: ephrin type-A receptor 4
• UniProt Protein Name: Ephrin type-A receptor 4
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: EPHA4
• UniProt Synonym Gene Names: HEK8; SEK; TYRO1; EK8; hEK8
• UniProt Entry Name: EPHA4_HUMAN

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]

Uniprot Description

EphA4: a tyrosine kinase receptor of the Eph family. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in hindbrain pattern formation. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels.

Protein type: Membrane protein, integral; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Kinase, protein; Protein kinase, TK; TK group; Eph family

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: axon; cytoplasm; dendrite; early endosome membrane; integral to plasma membrane; plasma membrane

Molecular Function: GPI-linked ephrin receptor activity; PH domain binding; protein binding; protein kinase activity; transmembrane-ephrin receptor activity

Biological Process: corticospinal tract morphogenesis; ephrin receptor signaling pathway; motor axon guidance; negative regulation of axon regeneration; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; regulation of astrocyte differentiation; regulation of axonogenesis; regulation of GTPase activity