DARC Blocking Peptide | ACKR1 blocking peptide

DARC Blocking Peptide (N-term)

• Gene Names: ACKR1; FY; Dfy; GPD; DARC; GpFy; CCBP1; CD234; WBCQ1
• Synonyms: DARC; DARC Blocking Peptide (N-term); Atypical chemokine receptor 1; Duffy antigen/chemokine receptor; Fy glycoprotein; GpFy; Glycoprotein D; Plasmodium vivax receptor; CD234; ACKR1; FY; GPD; ACKR1 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 12-25 of HUMAN ACKR1
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 336

• Cellular Location: Early endosome. Recycling endosome. Membrane; Multi-pass membrane protein. Note: Predominantly localizes to endocytic vesicles, and upon stimulation by the ligand is internalized via caveolae. Once internalized, the ligand dissociates from the receptor, and is targeted to degradation while the receptor is recycled back to the cell membrane
• Tissue Location: Found in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along postcapillary venules throughout the body, except in the adult liver. Erythroid cells and postcapillary venule endothelium are the principle tissues expressing duffy. Fy(-A-B) individuals do not express duffy in the bone marrow, however they do, in postcapillary venule endothelium
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ACKR1 blocking peptide

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Has a promiscuous chemokine-binding profile, interacting with inflammatory chemokines of both the CXC and the CC subfamilies but not with homeostatic chemokines. Acts as a receptor for chemokines including CCL2, CCL5, CCL7, CCL11, CCL13, CCL14, CCL17, CXCL5, CXCL6, IL8/CXCL8, CXCL11, GRO, RANTES, MCP-1, TARC and also for the malaria parasites P.vivax and P.knowlesi. May regulate chemokine bioavailability and, consequently, leukocyte recruitment through two distinct mechanisms: when expressed in endothelial cells, it sustains the abluminal to luminal transcytosis of tissue-derived chemokines and their subsequent presentation to circulating leukocytes; when expressed in erythrocytes, serves as blood reservoir of cognate chemokines but also as a chemokine sink, buffering potential surges in plasma chemokine levels.

NCBI and Uniprot Product Information

• NCBI GI #: 42822887
• NCBI GeneID: 2532
• NCBI Accession #: NP_002027.2
• NCBI GenBank Nucleotide #: NM_002036.3
• UniProt Accession #: Q16570; O75898; Q16300; Q8WWE3; Q9UJP0; Q9UKZ5; Q9UKZ6; Q9UQE1; A8YPG5
• Molecular Weight: 35,678 Da
• NCBI Official Full Name: atypical chemokine receptor 1 isoform b
• NCBI Official Synonym Full Names: atypical chemokine receptor 1 (Duffy blood group)
• NCBI Official Symbol: ACKR1
• NCBI Official Synonym Symbols: FY; Dfy; GPD; DARC; GpFy; CCBP1; CD234; WBCQ1
• NCBI Protein Information: atypical chemokine receptor 1
• UniProt Protein Name: Atypical chemokine receptor 1
• Protein Family: Darcynin
• UniProt Gene Name: ACKR1
• UniProt Synonym Gene Names: DARC; FY; GPD; GpFy
• UniProt Entry Name: ACKR1_HUMAN

NCBI Description

The protein encoded by this gene is a glycosylated membrane protein and a non-specific receptor for several chemokines. The encoded protein is the receptor for the human malarial parasites Plasmodium vivax and Plasmodium knowlesi. Polymorphisms in this gene are the basis of the Duffy blood group system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DARC: Non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi. Belongs to the G-protein coupled receptor Duffy family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; GPCR, Duffy family; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 1q21-q22

Cellular Component: plasma membrane

Molecular Function: C-C chemokine binding; receptor activity; transmembrane receptor activity

Biological Process: inflammatory response; regulation of chemokine production

Disease: Blood Group, Duffy System; Malaria, Susceptibility To; White Blood Cell Count Quantitative Trait Locus 1