NAMPT blocking peptide

NAMPT Antibody (Center) Blocking Peptide

• Gene Names: NAMPT; VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
• Synonyms: NAMPT; NAMPT Antibody (Center) Blocking Peptide; Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt; Pre-B-cell colony-enhancing factor 1; Pre-B cell-enhancing factor; Visfatin; PBEF; PBEF1; NAMPT blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Center region of human NAMPT. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 491

• Cellular Location: Nucleus. Cytoplasm. Secreted. Note: Under non-inflammatory conditions, visfatin predominantly exhibits a granular pattern within the nucleus. Secreted by endothelial cells upon IL-1beta stimulation. Abundantly secreted in milk, reaching 100-fold higher concentrations compared to maternal serum
• Tissue Location: Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for NAMPT blocking peptide

Catalyzes the condensation of nicotinamide with 5- phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 1172027
• NCBI GeneID: 10135
• UniProt Accession #: P43490; Q3KQV0; Q8WW95; A4D0Q9; A4D0R0
• Molecular Weight: 55,521 Da
• NCBI Official Full Name: Nicotinamide phosphoribosyltransferase
• NCBI Official Synonym Full Names: nicotinamide phosphoribosyltransferase
• NCBI Official Symbol: NAMPT
• NCBI Official Synonym Symbols: VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
• NCBI Protein Information: nicotinamide phosphoribosyltransferase
• UniProt Protein Name: Nicotinamide phosphoribosyltransferase
• Protein Family: Nicotinamide phosphoribosyltransferase
• UniProt Gene Name: NAMPT
• UniProt Synonym Gene Names: PBEF; PBEF1; NAmPRTase; Nampt; Pre-B cell-enhancing factor
• UniProt Entry Name: NAMPT_HUMAN

NCBI Description

This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011]

Uniprot Description

PBEF: an ubiquitous protein originally described as a cytokine which acts on early B-lineage precursor cells. Other reports indicate that PBEF is not a cytokine-like secreted protein but an intracellular protein associated with the cell cycle. Contains a catalytically active nicotinate phosphoribosyltransferase domain, an enzyme involved in nicotinamide adenine dinucleotide (NAD) biosynthesis.

Protein type: EC 2.4.2.12; Cell cycle regulation; Cytokine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Transferase

Chromosomal Location of Human Ortholog: 7q22.3

Cellular Component: cell junction; cytosol; nucleoplasm

Molecular Function: nicotinamide phosphoribosyltransferase activity; protein binding

Biological Process: cell-cell signaling; circadian regulation of gene expression; circadian rhythm; insulin receptor signaling pathway; nicotinamide metabolic process; positive regulation of cell proliferation; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; signal transduction