GNB2L1 blocking peptide

GNB2L1 Antibody (N-term) Blocking Peptide

• Gene Names: RACK1; H12.3; HLC-7; PIG21; GNB2L1; Gnb2-rs1
• Synonyms: GNB2L1; GNB2L1 Antibody (N-term) Blocking Peptide; Guanine nucleotide-binding protein subunit beta-2-like 1; Cell proliferation-inducing gene 21 protein; Guanine nucleotide-binding protein subunit beta-like protein 123; Human lung cancer oncogene 7 protein; HLC-7; Receptor for activated C kinase; Receptor of activated protein kinase C 1; RACK1; N-terminally processed; GNB2L1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human GNB2L1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 317

• Cellular Location: Cell membrane; Peripheral membrane protein. Cytoplasm. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton Nucleus. Perikaryon. Cell projection, dendrite. Cell projection, phagocytic cup. Note: Recruited to the plasma membrane through interaction with KRT1 which binds to membrane-bound ITGB1. Also associated with the membrane in oncogene-transformed cells. PKC activation induces translocation from the perinuclear region to the cell periphery. In the brain, detected mainly in cell bodies and dendrites with little expression in axonal fibers or nuclei. Localized to phagocytic cups following infection by Y.pestis
• Tissue Location: In the liver, expressed at higher levels in activated hepatic stellate cells than in hepatocytes or Kupffer cells. Up-regulated in hepatocellular carcinomas and in the adjacent non-tumor liver tissue.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for GNB2L1 blocking peptide

Involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression. Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand- independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration. Binds to Y.pseudotuberculosis yopK which leads to inhibition of phagocytosis and survival of bacteria following infection of host cells. Enhances phosphorylation of HIV-1 Nef by PKCs. Promotes migration of breast carcinoma cells by binding to and activating RHOA.

NCBI and Uniprot Product Information

• NCBI GI #: 5174447
• NCBI GeneID: 10399
• NCBI Accession #: NP_006089
• NCBI GenBank Nucleotide #: NM_006098.4
• UniProt Accession #: P63244; P25388; P99049; Q53HU2; Q5J8M6; Q5VLR4; Q6FH47; B3KTJ0; D3DWS0
• Molecular Weight: 35,077 Da
• NCBI Official Full Name: receptor of activated protein C kinase 1
• NCBI Official Synonym Full Names: receptor for activated C kinase 1
• NCBI Official Symbol: RACK1
• NCBI Official Synonym Symbols: H12.3; HLC-7; PIG21; GNB2L1; Gnb2-rs1
• NCBI Protein Information: receptor of activated protein C kinase 1
• UniProt Protein Name: Receptor of activated protein C kinase 1
• UniProt Gene Name: RACK1
• UniProt Synonym Gene Names: HLC-7
• UniProt Entry Name: RACK1_HUMAN

Uniprot Description

RACK1: a G-beta like protein containing 7 WD domains. A presumptive scaffold protein, linking protein kinase C to its substrates. Binds inositol 1,4,5-trisphosphate receptors and regulates Ca2+ release by enhancing inositol 1,4,5-trisphosphate receptor binding affinity for inositol 1,4,5-trisphosphate. An insulin-like growth factor 1 receptor-interacting protein that can regulate IGF-1-mediated Akt activation and protection from cell death.

Protein type: G protein, heterotrimeric beta; Adaptor/scaffold; G protein; G protein, heterotrimeric; Nuclear receptor co-regulator; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: cell soma; cytoplasm; cytosol; dendrite; midbody; mitochondrion; nucleus; perinuclear region of cytoplasm; phagocytic cup; small ribosomal subunit

Molecular Function: caspase activator activity; enzyme binding; ion channel inhibitor activity; protein binding; protein complex scaffold; protein homodimerization activity; protein kinase C binding; protein phosphatase binding; protein tyrosine kinase inhibitor activity; receptor tyrosine kinase binding; SH2 domain binding

Biological Process: caspase activation; negative regulation of cell growth; negative regulation of peptidyl-serine phosphorylation; negative regulation of phagocytosis; negative regulation of protein kinase B signaling cascade; negative regulation of translation; negative regulation of Wnt receptor signaling pathway; positive regulation of apoptosis; positive regulation of cAMP catabolic process; positive regulation of cell migration; positive regulation of cyclic-nucleotide phosphodiesterase activity; positive regulation of Golgi to plasma membrane protein transport; positive regulation of GTPase activity; positive regulation of mitochondrial depolarization; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein amino acid phosphorylation; positive regulation of protein homooligomerization; regulation of cell cycle; regulation of cell division; regulation of protein localization