BRD4 blocking peptide

BRD4 Antibody (C-term) Blocking Peptide

• Gene Names: BRD4; CAP; MCAP; HUNK1; HUNKI
• Synonyms: BRD4; BRD4 Antibody (C-term) Blocking Peptide; Bromodomain-containing protein 4; Protein HUNK1; HUNK1; BRD4 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human BRD4. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 1362

• Cellular Location: Nucleus. Chromosome. Note: Associates with acetylated chromatin. Released from chromatin upon deacetylation of histones that can be triggered by different signals such as activation of the JNK pathway or nocodazole treatment
• Tissue Location: Ubiquitously expressed.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for BRD4 blocking peptide

Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P- TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P- TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo (PubMed:22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters.

NCBI and Uniprot Product Information

• NCBI GI #: 20141192
• NCBI GeneID: 23476
• UniProt Accession #: O60885; O60433; Q4G0X8; Q86YS8; Q96PD3
• Molecular Weight: 88,289 Da
• NCBI Official Full Name: Bromodomain-containing protein 4
• NCBI Official Synonym Full Names: bromodomain containing 4
• NCBI Official Symbol: BRD4
• NCBI Official Synonym Symbols: CAP; MCAP; HUNK1; HUNKI
• NCBI Protein Information: bromodomain-containing protein 4
• UniProt Protein Name: Bromodomain-containing protein 4
• Protein Family: Bromodomain-containing protein
• UniProt Gene Name: BRD4
• UniProt Synonym Gene Names: HUNK1
• UniProt Entry Name: BRD4_HUMAN

NCBI Description

The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

BRD4: an atypical protein kinase of the Brd family. Contains a double bromodomain. Binds to chromatin and regulates cell cycle progression at multiple stages. Associates with chromosomes during mitosis. The cognate gene is the chromosome 19 target of translocation t(15;19)(q13;p13.1), resulting in the BRD4-NUT oncogene identified in a lethal carcinoma of young people. Binds to papillomavirus E2 protein and is required for its transforming ability. Two alternatively spliced isoforms have been described.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, atypical; ATYPICAL group; BRD family

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: condensed nuclear chromosome; cytoplasm; nucleoplasm; nucleus

Molecular Function: chromatin binding; p53 binding; protein binding

Biological Process: G1/S-specific transcription in mitotic cell cycle; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of RNA elongation from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; regulation of inflammatory response