DACT1 blocking peptide

DACT1 Antibody (N-term) Blocking peptide

• Gene Names: DACT1; DPR1; FRODO; HDPR1; DAPPER; THYEX3; DAPPER1
• Synonyms: DACT1; DACT1 Antibody (N-term) Blocking peptide; Dapper homolog 1; hDPR1; Dapper antagonist of catenin 1; Hepatocellular carcinoma novel gene 3 protein; DPR1; HNG3; DACT1 blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 836

• Cellular Location: Cytoplasm. Nucleus. Cell junction, synapse. Note: Shuttles between the nucleus and the cytoplasm. Seems to be nuclear in the absence of Wnt signaling and to translocate to the cytoplasm in its presence
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for DACT1 blocking peptide

Involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins. The activation/inhibition of Wnt signaling may depend on the phosphorylation status. Proposed to regulate the degradation of CTNNB1/beta-catenin, thereby modulating the transcriptional activation of target genes of the Wnt signaling pathway. Its function in stabilizing CTNNB1 may involve inhibition of GSK3B activity. Promotes the membrane localization of CTNNB1. The cytoplasmic form can induce DVL2 degradation via a lysosome- dependent mechanism; the function is inhibited by PKA-induced binding to 14-3-3 proteins, such as YWHAB. Seems to be involved in morphogenesis at the primitive streak by regulating VANGL2 and DVL2; the function seems to be independent of canonical Wnt signaling and rather involves the non-canonical Wnt/planar cell polarity (PCP) pathway (By similarity). The nuclear form may prevent the formation of LEF1:CTNNB1 complex and recruit HDAC1 to LEF1 at target gene promoters to repress transcription thus antagonizing Wnt signaling. May be involved in positive regulation of fat cell differentiation. During neuronal differentiation may be involved in excitatory synapse organization, and dendrite formation and establishment of spines.

NCBI and Uniprot Product Information

• NCBI GI #: 24158478
• NCBI GeneID: 51339
• NCBI Accession #: NP_057735.2
• NCBI GenBank Nucleotide #: NM_016651.5
• UniProt Accession #: Q9NYF0; Q86TY0; A8MYJ2
• Molecular Weight: 86,212 Da
• NCBI Official Full Name: dapper homolog 1 isoform 1
• NCBI Official Synonym Full Names: dishevelled binding antagonist of beta catenin 1
• NCBI Official Symbol: DACT1
• NCBI Official Synonym Symbols: DPR1; FRODO; HDPR1; DAPPER; THYEX3; DAPPER1
• NCBI Protein Information: dapper homolog 1
• UniProt Protein Name: Dapper homolog 1
• Protein Family: Dapper
• UniProt Gene Name: DACT1
• UniProt Synonym Gene Names: DPR1; HNG3; hDPR1
• UniProt Entry Name: DACT1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the dapper family, characterized by the presence of PDZ-binding motif at the C-terminus. It interacts with, and positively regulates dishevelled-mediated signaling pathways during development. Depletion of this mRNA from xenopus embryos resulted in loss of notochord and head structures, and mice lacking this gene died shortly after birth from severe posterior malformations. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

DACT1: Positively regulates DVL2-mediated signaling pathways during development. Binds to DVL2 and impedes the degradation of CTNNB1/beta-catenin, thereby enhancing the transcriptional activation of target genes of the Wnt signaling pathway. Interacts with DVL1 and DVL2. Belongs to the dapper family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: beta-catenin destruction complex; cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: beta-catenin binding; histone deacetylase binding; protein binding; protein kinase A binding; protein kinase C binding

Biological Process: embryonic hindgut morphogenesis; negative regulation of JNK cascade; negative regulation of protein binding; negative regulation of transcription from RNA polymerase II promoter; negative regulation of Wnt receptor signaling pathway; neural tube development; positive regulation of protein binding; positive regulation of protein catabolic process; positive regulation of Wnt receptor signaling pathway; regulation of protein stability