Cblb blocking peptide

Mouse Cblb Antibody (C-term) Blocking Peptide

• Gene Names: Cblb; Cbl-b; AI429560; AI851073
• Synonyms: Cblb; Mouse Cblb Antibody (C-term) Blocking Peptide; E3 ubiquitin-protein ligase CBL-B; 632-; Casitas B-lineage lymphoma proto-oncogene b; SH3-binding protein CBL-B; Signal transduction protein CBL-B; Cblb blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 982

• Cellular Location: Cytoplasm. Note: In adipocytes, translocates to the plasma membrane upon insulin stimulation
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Cblb blocking peptide

E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B- cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T- cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.

NCBI and Uniprot Product Information

• NCBI GI #: 341940556
• NCBI GeneID: 208650
• UniProt Accession #: Q3TTA7; E9QMY2
• Molecular Weight: 91,524 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase CBL-B
• NCBI Official Synonym Full Names: Casitas B-lineage lymphoma b
• NCBI Official Symbol: Cblb
• NCBI Official Synonym Symbols: Cbl-b; AI429560; AI851073
• NCBI Protein Information: E3 ubiquitin-protein ligase CBL-B
• UniProt Protein Name: E3 ubiquitin-protein ligase CBL-B
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: Cblb
• UniProt Entry Name: CBLB_MOUSE

Uniprot Description

Cbl-b: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B- cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T- cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. May also be involved in EGFR ubiquitination and internalization. Interacts with SH3 domain-containing proteins LCK, CRK and SORBS1. Interacts with LCP2 and ZAP70. May interact with CBL. Interacts with SH3 domain-containing proteins VAV1, FYN, FGR, PLCG1, GRB2, CRKL, PIK3R1 and SH3KBP1/CIN85. Identified in heterotrimeric complexes with SH3KBP1/CIN85, CD2AP and ARHGEF7, where one CBLB peptide binds two copies of the other protein. Interacts with poly-ubiquitinated proteins. Dimerization is required for the binding of poly-ubiquitin, but not for the binding of mono-ubiquitin. Expressed in placenta, heart, lung, kidney, spleen, ovary and testis, as well as fetal brain and liver and hematopoietic cell lines, but not in adult brain, liver, pancreas, salivary gland, or skeletal muscle. Present in lymphocytes. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Calcium-binding; Ubiquitin conjugating system; EC 6.3.2.-; Adaptor/scaffold; Ligase; Ubiquitin ligase

Cellular Component: cytoplasm; lipid raft; nucleoplasm

Molecular Function: protein binding; protein kinase binding; ubiquitin-protein ligase activity

Biological Process: immune response; negative regulation of alpha-beta T cell proliferation; negative regulation of T cell receptor signaling pathway; positive regulation of protein catabolic process; positive regulation of T cell anergy; signal transduction; T cell activation