Rabbit anti-Mouse Cblb Polyclonal Antibody | anti-Cblb antibody

Cblb, CT (Cblb, E3 ubiquitin-protein ligase CBL-B, Casitas B-lineage lymphoma proto-oncogene b, SH3-binding protein CBL-B, Signal transduction protein CBL-B) (PE)

Cat. Num. AAA6281133

• Gene Names: Cblb; Cbl-b; AI429560; AI851073
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Cblb; Polyclonal Antibody; CT (Cblb; E3 ubiquitin-protein ligase CBL-B; Casitas B-lineage lymphoma proto-oncogene b; SH3-binding protein CBL-B; Signal transduction protein CBL-B) (PE); Signal transduction protein CBL-B; anti-Cblb antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Cblb.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-Cblb antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 862-889 in the C-terminal region of mouse Cblb.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse liver tissue lysates (35ug/lane) usingMBS6001181. This demonstrates that MBS6001181 detected the Cblb protein (arrow).)

Related Product Information for anti-Cblb antibody

E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. May also be involved in EGFR ubiquitination and internalization.

Product Categories/Family for anti-Cblb antibody

Antibodies; Ubiquitin Ligases

References

1. Wilson, B.G., et al. Cancer Cell 18(4):316-328(2010). 2. Stromnes, I.M., et al. J. Clin. Invest. 120(10):3722-3734(2010). 3. Naramura, M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(37):16274-16279(2010). 4. Teh, C.E., et al. Proc. Natl. Acad. Sci. U.S.A. 107(33):14709-14714(2010). 5. Huang, H., et al. Immunity 33(1):60-70(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 118344448
• NCBI GeneID: 208650
• NCBI Accession #: NP_001028410.1
• NCBI GenBank Nucleotide #: NM_001033238.1
• UniProt Accession #: Q3TTA7; E9QMY2
• Molecular Weight: 109,092 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase CBL-B
• NCBI Official Synonym Full Names: Casitas B-lineage lymphoma b
• NCBI Official Symbol: Cblb
• NCBI Official Synonym Symbols: Cbl-b; AI429560; AI851073
• NCBI Protein Information: E3 ubiquitin-protein ligase CBL-B; SH3-binding protein CBL-B; signal transduction protein CBL-B; casitas B-lineage lymphoma proto-oncogene b
• UniProt Protein Name: E3 ubiquitin-protein ligase CBL-B
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: Cblb
• UniProt Entry Name: CBLB_MOUSE