POLR2B blocking peptide

POLR2B Antibody (Center) Blocking Peptide

• Gene Names: POLR2B; RPB2; POL2RB; hRPB140
• Synonyms: POLR2B; POLR2B Antibody (Center) Blocking Peptide; DNA-directed RNA polymerase II subunit RPB2; DNA-directed RNA polymerase II 140 kDa polypeptide; DNA-directed RNA polymerase II subunit B; RNA polymerase II subunit 2; RNA polymerase II subunit B2; POLR2B blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1174

• Cellular Location: Nucleus.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for POLR2B blocking peptide

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 401012
• NCBI GeneID: 5431
• UniProt Accession #: P30876; Q8IZ61; A8K1A8; AAB23139
• Molecular Weight: 133,897 Da
• NCBI Official Full Name: DNA-directed RNA polymerase II subunit RPB2
• NCBI Official Synonym Full Names: RNA polymerase II subunit B
• NCBI Official Symbol: POLR2B
• NCBI Official Synonym Symbols: RPB2; POL2RB; hRPB140
• NCBI Protein Information: DNA-directed RNA polymerase II subunit RPB2
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB2
• Protein Family: DNA-directed RNA polymerase
• UniProt Gene Name: POLR2B
• UniProt Entry Name: RPB2_HUMAN

NCBI Description

This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

POLR2B: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. Belongs to the RNA polymerase beta chain family.

Protein type: RNA splicing; RNA-binding; Nucleotide Metabolism - pyrimidine; Transferase; Transcription initiation complex; RNA processing; EC 2.7.7.6; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: DNA-directed RNA polymerase II, core complex; membrane; nuclear chromosome, telomeric region; nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: fibroblast growth factor receptor signaling pathway; gene expression; mRNA capping; nuclear mRNA splicing, via spliceosome; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; RNA-mediated gene silencing; snRNA transcription from RNA polymerase II promoter; somatic stem cell maintenance; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair