Plk3 blocking peptide

Mouse Plk3 Antibody (N-term) Blocking Peptide

• Gene Names: Plk3; Cnk; Fnk; PRK; PLK-3
• Synonyms: Plk3; Mouse Plk3 Antibody (N-term) Blocking Peptide; Serine/threonine-protein kinase PLK3; Cytokine-inducible serine/threonine-protein kinase; FGF-inducible kinase; Polo-like kinase 3; PLK-3; Cnk; Fnk; Plk3 blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 631

• Cellular Location: Cytoplasm. Nucleus. Nucleus, nucleolus. Golgi apparatus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note: Translocates to the nucleus upon cisplatin treatment. Localizes to the Golgi apparatus during interphase (By similarity).
• Tissue Location: Expressed in skin.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Plk3 blocking peptide

Serine/threonine-protein kinase involved in cell cycle regulation, response to stress and Golgi disassembly. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/TP53, p73/TP73, PTEN, TOP2A and VRK1. Involved in cell cycle regulation: required for entry into S phase and cytokinesis. Phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Plays a key role in response to stress: rapidly activated upon stress stimulation, such as ionizing radiation, reactive oxygen species (ROS), hyperosmotic stress, UV irradiation and hypoxia. Involved in DNA damage response and G1/S transition checkpoint by phosphorylating CDC25A, p53/TP53 and p73/TP73. Phosphorylates p53/TP53 in response to reactive oxygen species (ROS), thereby promoting p53/TP53-mediated apoptosis. Phosphorylates CHEK2 in response to DNA damage, promoting the G2/M transition checkpoint. Phosphorylates the transcription factor p73/TP73 in response to DNA damage, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates HIF1A and JUN is response to hypoxia. Phosphorylates ATF2 following hyperosmotic stress in corneal epithelium. Also involved in Golgi disassembly during the cell cycle: part of a MEK1/MAP2K1-dependent pathway that induces Golgi fragmentation during mitosis by mediating phosphorylation of VRK1. May participate in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation, via its interaction with CIB1.

NCBI and Uniprot Product Information

• NCBI GI #: 13878440
• NCBI GeneID: 12795
• UniProt Accession #: Q60806; Q60822; Q9R009
• Molecular Weight: 71,845 Da
• NCBI Official Full Name: Serine/threonine-protein kinase PLK3
• NCBI Official Synonym Full Names: polo-like kinase 3
• NCBI Official Symbol: Plk3
• NCBI Official Synonym Symbols: Cnk; Fnk; PRK; PLK-3
• NCBI Protein Information: serine/threonine-protein kinase PLK3
• UniProt Protein Name: Serine/threonine-protein kinase PLK3
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: Plk3
• UniProt Synonym Gene Names: Cnk; Fnk; PLK-3
• UniProt Entry Name: PLK3_MOUSE

NCBI Description

This gene encodes a member of the highly conserved polo-like kinase family of serine/threonine kinases. Members of this family are characterized by an amino-terminal catalytic domain and a carboxy-terminal bipartite polo box domain that functions as a substrate-binding motif and a cellular localization signal. Polo-like kinases have primarily been implicated in cell cycle regulation. In mouse, this protein that has been reported to localize to the nucleolus during interphase but is undetectable during mitosis, following nucleolus dissociation during prophase. The protein relocalizes to the nucleolus just prior to cytokinesis and peak levels are detected during G1 of interphase. This gene has been implicated in regulation of entry into S phase, with RNAi-induced depletion resulting in failure to re-enter the cell cycle. Mice deficient for this gene exhibit increased weight and tumor development at advanced age. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

PLK3: a kinase of the PLK family. Phosphorylated and activated following DNA damage or mitotic spindle disruption. Interacts with Chk2 and the interaction is enhanced upon DNA damage. Apparent substrates include Chk2 and p53. Plays an important role in regulating microtubule dynamics and centrosomal function. Deregulated expression of Plk3 results in cell cycle arrest and apoptosis.

Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Nucleolus; Kinase, protein; EC 2.7.11.21; Other group; PLK family

Cellular Component: cell soma; centrosome; chromatin; cytoplasm; dendrite; Golgi stack; intracellular; nucleolus; nucleus

Molecular Function: p53 binding; protein binding; protein serine/threonine kinase activity

Biological Process: cytoplasmic microtubule organization and biogenesis; G1/S transition of mitotic cell cycle; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; protein kinase B signaling cascade; regulation of cell division; regulation of cytokinesis; response to DNA damage stimulus; response to osmotic stress; response to radiation; response to reactive oxygen species