SNX6 blocking peptide

SNX6 Antibody (N-term) Blocking Peptide

• Gene Names: SNX6; TFAF2; MSTP010
• Synonyms: SNX6; SNX6 Antibody (N-term) Blocking Peptide; Sorting nexin-6; TRAF4-associated factor 2; N-terminally processed; SNX6 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human SNX6. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 406

• Cellular Location: Early endosome. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side Cytoplasmic vesicle. Cytoplasm. Nucleus Note: Interaction with SNX1 or SNX2 promotes location at endosome membranes (PubMed:19935774). Only a minor proportion is seen in the nucleus.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SNX6 blocking peptide

Involved in several stages of intracellular trafficking. Interacts with membranes phosphatidylinositol 3,4-bisphosphate and/or phosphatidylinositol 4,5-bisphosphate (Probable). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex (PubMed:19935774). The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC) (Probable). Does not have in vitro vesicle-to-membrane remodeling activity (PubMed:23085988). Involved in retrograde endosome-to-TGN transport of lysosomal enzyme receptor IGF2R (PubMed:17148574). May function as link between transport vesicles and dynactin (Probable). Negatively regulates retrograde transport of BACE1 from the cell surface to the trans-Golgi network (PubMed:20354142). Involved in E-cadherin sorting and degradation; inhibits PIP5K1C isoform 3-mediated E-cadherin degradation (PubMed:24610942). In association with GIT1 involved in EGFR degradation. Promotes lysosomal degradation of CDKN1B (By similarity). May contribute to transcription regulation (Probable).

NCBI and Uniprot Product Information

• NCBI GI #: 10720285
• NCBI GeneID: 58533
• UniProt Accession #: Q9UNH7; Q9Y449; C0H5W9
• Molecular Weight: 33,570 Da
• NCBI Official Full Name: Sorting nexin-6
• NCBI Official Synonym Full Names: sorting nexin 6
• NCBI Official Symbol: SNX6
• NCBI Official Synonym Symbols: TFAF2; MSTP010
• NCBI Protein Information: sorting nexin-6
• UniProt Protein Name: Sorting nexin-6
• Protein Family: Sorting nexin
• UniProt Gene Name: SNX6
• UniProt Entry Name: SNX6_HUMAN

NCBI Description

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein associates with the long isoform of the leptin receptor, the transforming growth factor-beta family of receptor serine-threonine kinases, and with receptor tyrosine kinases for platelet-derived growth factor, insulin, and epidermal growth factor. This protein may form oligomeric complexes with family member proteins through interactions of both the PX domain and the coiled coil regions of the molecules. Translocation of this protein from the cytoplasm to the nucleus occurs after binding to proviral integration site 1 protein. This gene results in two transcripts encoding two distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

SNX6: May be involved in several stages of intracellular trafficking. Promotes lysosomal degradation of CDKN1B. Plays a role in retrograde protein transport from endosomes to the trans-Golgi network. May function as link between transport vesicles and dynactin. Negatively regulates retrograde transport of BACE1 from the cell surface to the trans-Golgi network. May contribute to transcription regulation. Belongs to the sorting nexin family.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 14q13.1

Cellular Component: cytoplasm; early endosome membrane; extrinsic to membrane; retromer complex

Molecular Function: phosphoinositide binding; protein binding; protein homodimerization activity

Biological Process: endocytosis; intracellular protein transport; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; retrograde transport, endosome to Golgi; vesicle organization and biogenesis