CIDEA blocking peptide

CIDEA Antibody (C-term) Blocking peptide

• Gene Names: CIDEA; CIDE-A
• Synonyms: CIDEA; CIDEA Antibody (C-term) Blocking peptide; Cell death activator CIDE-A; Cell death-inducing DFFA-like effector A; CIDEA blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of CIDEA. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 219

• Cellular Location: Lipid droplet. Nucleus. Note: Enriched at lipid droplet contact sites. Has been shown to localize to mitonchondria, where it could interact with UCP1 and hence inhibit UCP1 uncoupling activity (By similarity). These data could not be confirmed
• Tissue Location: Expressed in omental and subcutaneous adipose tissue (at protein level).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CIDEA blocking peptide

Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter (By similarity). Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear.

NCBI and Uniprot Product Information

• NCBI GI #: 9087117
• NCBI GeneID: 1149
• UniProt Accession #: O60543; Q6UPR7; B0YIY7
• Molecular Weight: 24,687 Da
• NCBI Official Full Name: Cell death activator CIDE-A
• NCBI Official Synonym Full Names: cell death-inducing DFFA-like effector a
• NCBI Official Symbol: CIDEA
• NCBI Official Synonym Symbols: CIDE-A
• NCBI Protein Information: cell death activator CIDE-A
• UniProt Protein Name: Cell death activator CIDE-A
• Protein Family: Cell death activator
• UniProt Gene Name: CIDEA
• UniProt Entry Name: CIDEA_HUMAN

NCBI Description

This gene encodes the homolog of the mouse protein Cidea that has been shown to activate apoptosis. This activation of apoptosis is inhibited by the DNA fragmentation factor DFF45 but not by caspase inhibitors. Mice that lack functional Cidea have higher metabolic rates, higher lipolysis in brown adipose tissue and higher core body temperatures when subjected to cold. These mice are also resistant to diet-induced obesity and diabetes. This suggests that in mice this gene product plays a role in thermogenesis and lipolysis. Alternatively spliced transcripts have been identified. [provided by RefSeq, Aug 2010]

Uniprot Description

CIDEA: Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter. Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear. In omental and subcutaneous adipose tissue of obese patients matched for BMI, expression levels correlate with insulin sensitivity. Expression is increased 5-6 fold in the group of patients with high insulin sensitivity, compared to the insulin- resistant group. This observation is consistent with the idea that triglyceride storage in adipocytes plays an important role in sequestering triglycerides and fatty acids away from the circulation and peripheral tissues, thus enhancing insulin sensitivity in liver and muscle.

Protein type: Mitochondrial; Apoptosis

Chromosomal Location of Human Ortholog: 18p11.21|18

Cellular Component: cytoplasm; lipid particle; mitochondrial envelope; mitochondrion; nucleus

Molecular Function: protein homodimerization activity

Biological Process: cell death; lipid metabolic process; negative regulation of cytokine secretion; negative regulation of lipid catabolic process; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of tumor necrosis factor production; regulation of apoptosis; sequestering of lipid; thermoregulation