Western Blot (WB)
(Western blot analysis of PLCG2 (Phospho-Tyr759) using H2O2 treated 293 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Rabbit PLCG2 Polyclonal Antibody | anti-PLCG2 antibody
Phospho-PLCG2 (Tyr759) Antibody
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
IF/ICC: 1:100-1:500
Peptide ELISA: 1:20,000-1:40,000
Western Blot (WB)
(Western blot analysis of PLCG2 (Phospho-Tyr759) using H2O2 treated 293 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Western Blot (WB)
(Western blot analysis of PLCG2 (Phospho-Tyr759) using H2O2 treated 293 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG), using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. [provided by RefSeq, Sep 2011]
Uniprot Description
PLCG2: a calcium dependent phosphatidylinositol-specific phospholipase C. The activated enzyme produces the second messenger molecules diacylglycerol and inositol 1,4,5-trisphosphate. It is a crucial enzyme in transmembrane signaling. Phosphorylated and activated by tyrosine kinases in response to signaling through a variety of growth factor receptors and immune system receptors.
Protein type: Motility/polarity/chemotaxis; Carbohydrate Metabolism - inositol phosphate; EC 3.1.4.11; Phospholipase
Chromosomal Location of Human Ortholog: 16q24.1
Cellular Component: plasma membrane; cytosol
Molecular Function: protein binding; signal transducer activity; phospholipase C activity; phosphoinositide phospholipase C activity
Biological Process: platelet activation; Wnt receptor signaling pathway; inositol phosphate metabolic process; phospholipid catabolic process; calcium-mediated signaling; response to lipopolysaccharide; T cell receptor signaling pathway; B cell receptor signaling pathway; activation of store-operated calcium channel activity; inositol trisphosphate biosynthetic process; regulation of gene expression; B cell differentiation; release of sequestered calcium ion into cytosol; negative regulation of programmed cell death; phosphatidylinositol biosynthetic process; innate immune response; follicular B cell differentiation; blood coagulation
Disease: Autoinflammation, Antibody Deficiency, And Immune Dysregulation, Plcg2-associated; Familial Cold Autoinflammatory Syndrome 3
Research Articles on PLCG2
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Product Notes
The PLCG2 plcg2 (Catalog #AAA9614019) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-PLCG2 (Tyr759) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's PLCG2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA). WB: 1:1000-3000 IF/ICC: 1:100-1:500 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the PLCG2 plcg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLCG2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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