ROBLD3 Blocking Peptide | LAMTOR2 blocking peptide

ROBLD3 Antibody (Center) Blocking peptide

• Gene Names: LAMTOR2; p14; ENDAP; ROBLD3; HSPC003; MAPBPIP; MAPKSP1AP; Ragulator2
• Synonyms: ROBLD3; ROBLD3 Antibody (Center) Blocking peptide; Ragulator complex protein LAMTOR2; Endosomal adaptor protein p14; Late endosomal/lysosomal Mp1-interacting protein; Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2; Mitogen-activated protein-binding protein-interacting protein; MAPBP-interacting protein; Roadblock domain-containing protein 3; LAMTOR2; MAPBPIP; LAMTOR2 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Center region of ROBLD3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 125

• Cellular Location: Late endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for LAMTOR2 blocking peptide

As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2.

NCBI and Uniprot Product Information

• NCBI GI #: 12585246
• NCBI GeneID: 28956
• UniProt Accession #: Q9Y2Q5; Q5VY97; Q5VY98; Q5VY99
• Molecular Weight: 10,242 Da
• NCBI Official Full Name: Ragulator complex protein LAMTOR2
• NCBI Official Synonym Full Names: late endosomal/lysosomal adaptor, MAPK and MTOR activator 2
• NCBI Official Symbol: LAMTOR2
• NCBI Official Synonym Symbols: p14; ENDAP; ROBLD3; HSPC003; MAPBPIP; MAPKSP1AP; Ragulator2
• NCBI Protein Information: ragulator complex protein LAMTOR2
• UniProt Protein Name: Ragulator complex protein LAMTOR2
• Protein Family: Ragulator complex protein
• UniProt Gene Name: LAMTOR2
• UniProt Synonym Gene Names: MAPBPIP; ROBLD3; MAPBP-interacting protein
• UniProt Entry Name: LTOR2_HUMAN

NCBI Description

The product of this gene is highly conserved with a mouse protein associated with the cytoplasmic face of late endosomes and lysosomes. The mouse protein interacts with MAPK scaffold protein 1, a component of the mitogen-activated protein kinase pathway. In humans, a mutation in this gene has been associated with a primary immunodeficiency syndrome, and suggests a role for this protein in endosomal biogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

Uniprot Description

LAMTOR2: Adapter protein that enhances the efficiency of the MAP kinase cascade. Facilitates the activation of MAPKAPK2 . Heterodimer with MAP2K1IP1. Interacts with ERK and MEK1. Defects in ROBLD3 are the cause of immunodeficiency due to defect in MAPBP-interacting protein. This form of primary immunodeficiency syndrome includes congenital neutropenia, partial albinism, short stature and B-cell and cytotoxic T-cell deficiency. Two alternatively spliced isoforms of the human protein have been described.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: endosome membrane; late endosome; lysosomal membrane

Molecular Function: guanyl-nucleotide exchange factor activity; protein binding; protein complex scaffold

Biological Process: cell cycle arrest; cell growth; macroautophagy; MAPKKK cascade; positive regulation of TOR signaling pathway

Disease: Immunodeficiency Due To Defect In Mapbp-interacting Protein