LAMTOR2 recombinant protein

LAMTOR2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121202

• Gene Names: LAMTOR2; p14; ENDAP; ROBLD3; HSPC003; MAPBPIP; MAPKSP1AP; Ragulator2
• Purity: >97% as determined by SDS-PAGE
• Synonyms: LAMTOR2; LAMTOR2 Protein; Human; Recombinant (His Tag); Human LAMTOR2/ROBLD3/MAPBPIP Protein (His Tag); ENDAP Protein; HSPC003 Protein; MAPBPIP Protein; MAPKSP1AP Protein; p14 Protein; Ragulator2 Protein; ROBLD3 Protein; RP11-336K24.9 Protein; late endosomal/lysosomal adaptor; MAPK and MTOR activator 2; LAMTOR2 recombinant protein

• Host: E Coli
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser125

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human LAMTOR2 isoform 1 (Q9Y2Q5-1)(Met 1-Ser 125) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for LAMTOR2 recombinant protein

Background: Ragulator complex protein LAMTOR2, also known as Endosomal adaptor protein p14, Late endosomal/lysosomal Mp1-interacting protein, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2, Mitogen-activated protein-binding protein-interacting protein, Roadblock domain-containing protein 3, LAMTOR2, MAPBPIP, and ROBLD3, is a protein which belongs to the GAMAD family. LAMTOR2/ROBLD3 is a regulator of the TOR pathway, a signaling cascade that promotes cell growth in response to growth factors, energy levels, and amino acids. As part of the Ragulator complex, LAMTOR2/ROBLD3 recruits the Rag GTPases and the mTORC1 complex to lysosomes, a key step in the activation of the TOR signaling cascade by amino acids. LAMTOR2/ROBLD3 is an adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2. Defects in LAMTOR2 are the cause of immunodeficiency due to defects in MAPBP-interacting protein (ID-MAPBPIP). This form of primary immunodeficiency syndrome includes congenital neutropenia, partial albinism, short stature, and B-cell and cytotoxic T-cell deficiency.

Product Categories/Family for LAMTOR2 recombinant protein

MAPK-Erk Pathway

References

Schroeder B., et al., 2007, Traffic 8:1676-1686. Bohn G., et al., 2007, Nat. Med. 13:38-45. Sancak Y., et al., 2010, Cell 141:290-303. Burkard T.R., et al., 2011, BMC Syst. Biol. 5:17-17.

NCBI and Uniprot Product Information

• NCBI GI #: 223671906
• NCBI GeneID: 28956
• NCBI Accession #: NP_001138736.1
• NCBI GenBank Nucleotide #: NM_001145264.1
• UniProt Accession #: Q9Y2Q5; Q5VY97; Q5VY98; Q5VY99
• Molecular Weight: 10,242 Da
• NCBI Official Full Name: ragulator complex protein LAMTOR2 isoform 2
• NCBI Official Synonym Full Names: late endosomal/lysosomal adaptor, MAPK and MTOR activator 2
• NCBI Official Symbol: LAMTOR2
• NCBI Official Synonym Symbols: p14; ENDAP; ROBLD3; HSPC003; MAPBPIP; MAPKSP1AP; Ragulator2
• NCBI Protein Information: ragulator complex protein LAMTOR2; MAPBP-interacting protein; MAPKSP1 adaptor protein; endosomal adaptor protein p14; late endosomal/lysosomal Mp1-interacting protein; late endosomal/lysosomal adaptor and MAPK and MTOR activator 2; mitogen-activated prote
• UniProt Protein Name: Ragulator complex protein LAMTOR2
• Protein Family: Ragulator complex protein
• UniProt Gene Name: LAMTOR2
• UniProt Synonym Gene Names: MAPBPIP; ROBLD3; MAPBP-interacting protein
• UniProt Entry Name: LTOR2_HUMAN

NCBI Description

The product of this gene is highly conserved with a mouse protein associated with the cytoplasmic face of late endosomes and lysosomes. The mouse protein interacts with MAPK scaffold protein 1, a component of the mitogen-activated protein kinase pathway. In humans, a mutation in this gene has been associated with a primary immunodeficiency syndrome, and suggests a role for this protein in endosomal biogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

Uniprot Description

LAMTOR2: Adapter protein that enhances the efficiency of the MAP kinase cascade. Facilitates the activation of MAPKAPK2 . Heterodimer with MAP2K1IP1. Interacts with ERK and MEK1. Defects in ROBLD3 are the cause of immunodeficiency due to defect in MAPBP-interacting protein. This form of primary immunodeficiency syndrome includes congenital neutropenia, partial albinism, short stature and B-cell and cytotoxic T-cell deficiency. Two alternatively spliced isoforms of the human protein have been described.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: lysosomal membrane; late endosome

Molecular Function: protein binding; guanyl-nucleotide exchange factor activity; protein complex scaffold

Biological Process: activation of MAPKK activity; cell growth; positive regulation of TOR signaling pathway; positive regulation of GTPase activity

Disease: Immunodeficiency Due To Defect In Mapbp-interacting Protein