SMYD2 blocking peptide

SMYD2 Antibody (N-term) Blocking Peptide

• Gene Names: SMYD2; KMT3C; HSKM-B; ZMYND14
• Synonyms: SMYD2; SMYD2 Antibody (N-term) Blocking Peptide; N-lysine methyltransferase SMYD2; 211-; HSKM-B; Histone methyltransferase SMYD2; Lysine N-methyltransferase 3C; SET and MYND domain-containing protein 2; KMT3C; SMYD2 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human SMYD2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 433

• Cellular Location: Cytoplasm, cytosol. Nucleus
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SMYD2 blocking peptide

Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860'.

NCBI and Uniprot Product Information

• NCBI GI #: 90185234
• NCBI GeneID: 56950
• UniProt Accession #: Q9NRG4; Q4V765; Q5VSH9; Q96AI4; B2R9P9; I6L9H7
• Molecular Weight: 30,964 Da
• NCBI Official Full Name: N-lysine methyltransferase SMYD2
• NCBI Official Synonym Full Names: SET and MYND domain containing 2
• NCBI Official Symbol: SMYD2
• NCBI Official Synonym Symbols: KMT3C; HSKM-B; ZMYND14
• NCBI Protein Information: N-lysine methyltransferase SMYD2
• UniProt Protein Name: N-lysine methyltransferase SMYD2
• Protein Family: N-lysine methyltransferase
• UniProt Gene Name: SMYD2
• UniProt Synonym Gene Names: KMT3C
• UniProt Entry Name: SMYD2_HUMAN

NCBI Description

SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).[supplied by OMIM, Nov 2008]

Uniprot Description

SMYD2: Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Has also methyltransferase activity toward non-histone proteins such as p53/TP53 and RB1. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates 'Lys-860' of RB1/RB. Interacts with RNA polymerase II and HELZ. Interacts with SIN3A and HDAC1. Interacts (via MYND-type zinc finger) with EPB41L3. Interacts (via SET domain) with p53/TP53. Interacts with RB1 and HSP90AA1. Expression is repressed by CEBPA.

Protein type: Methyltransferase, protein lysine; EC 2.1.1.43; Methyltransferase

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: histone lysine N-methyltransferase activity (H3-K36 specific); histone-lysine N-methyltransferase activity; lysine N-methyltransferase activity; p53 binding; protein binding; protein-lysine N-methyltransferase activity

Biological Process: negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; peptidyl-lysine di-methylation; peptidyl-lysine mono-methylation; regulation of DNA damage response, signal transduction by p53 class mediator