Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (All lanes : Anti-APOL3 Antibody (N-Term) at 1:2000 dilutionLane 1: A549 whole cell lysateLane 2: HepG2 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human APOL3 Polyclonal Antibody | anti-APOL3 antibody

APOL3 Antibody (N-Term)

Gene Names
APOL3; CG121; CG12_1; APOLIII; apoL-III
Reactivity
Human
Applications
Western Blot, ELISA
Synonyms
APOL3; Polyclonal Antibody; APOL3 Antibody (N-Term); Apolipoprotein L3; Apolipoprotein L-III; ApoL-III; TNF-inducible protein CG12-1; CG12_1; Purified Rabbit Polyclonal Antibody (Pab); anti-APOL3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This APOL3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 57-90 amino acids from human APOL3.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
1.8 (varies by lot)
Sequence Length
402
Applicable Applications for anti-APOL3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:2000
Cellular Location
Cytoplasm.
Tissue Location
Widely expressed; the highest levels are in prostate, lung and placenta; also detected in kidney, bone marrow, spleen, thymus, spinal cord, adrenal gland, salivary gland, trachea and mammary gland; levels are low in brain, heart, fetal liver, pancreas and testis
Antigen Type
Synthesized Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-APOL3 Antibody (N-Term) at 1:2000 dilutionLane 1: A549 whole cell lysateLane 2: HepG2 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-APOL3 Antibody (N-Term) at 1:2000 dilutionLane 1: A549 whole cell lysateLane 2: HepG2 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-APOL3 antibody
May affect the movement of lipids in the cytoplasm or allow the binding of lipids to organelles.
Product Categories/Family for anti-APOL3 antibody
References
Horrevoets A.J.G.,et al.Blood 93:3418-3431(1999). Duchateau P.N.,et al.J. Lipid Res. 42:620-630(2001). Page N.M.,et al.Genomics 74:71-78(2001). Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004). Dunham I.,et al.Nature 402:489-495(1999).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
44278 Da
NCBI Official Full Name
Apolipoprotein L3
NCBI Official Synonym Full Names
apolipoprotein L3
NCBI Official Symbol
APOL3
NCBI Official Synonym Symbols
CG121; CG12_1; APOLIII; apoL-III
NCBI Protein Information
apolipoprotein L3
UniProt Protein Name
Apolipoprotein L3
Protein Family
UniProt Gene Name
APOL3
UniProt Synonym Gene Names
ApoL-III; CG12_1
UniProt Entry Name
APOL3_HUMAN

NCBI Description

This gene is a member of the apolipoprotein L gene family, and it is present in a cluster with other family members on chromosome 22. The encoded protein is found in the cytoplasm, where it may affect the movement of lipids, including cholesterol, and/or allow the binding of lipids to organelles. In addition, expression of this gene is up-regulated by tumor necrosis factor-alpha in endothelial cells lining the normal and atherosclerotic iliac artery and aorta. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]

Uniprot Description

APOL3: May affect the movement of lipids in the cytoplasm or allow the binding of lipids to organelles. Belongs to the apolipoprotein L family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: membrane

Molecular Function: lipid transporter activity; signal transducer activity

Biological Process: inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade

Research Articles on APOL3

Similar Products

Product Notes

The APOL3 apol3 (Catalog #AAA9217339) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The APOL3 Antibody (N-Term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's APOL3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:2000. Researchers should empirically determine the suitability of the APOL3 apol3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APOL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.