Western Blot (WB)
(All lanes : Anti-POTE Antibody (C-term L446) at 1:500 dilutionLane 1: human ovary lysateLane 2: human testis lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human POTE Polyclonal Antibody | anti-POTED antibody
POTE Antibody (C-term L446)
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Synonyms
POTE; Polyclonal Antibody; POTE Antibody (C-term L446); POTE ankyrin domain family member D; ANKRD26-like family B member 3; Ankyrin repeat domain-containing protein 21; Prostate; ovary; testis-expressed protein; Protein POTE; POTED; A26B3; ANKRD21; anti-POTED antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This POTE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438~468 amino acids from the C-term region of human POTE.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
584
Applicable Applications for anti-POTED antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
Application Notes
WB:~~1:500
IHC-P:~~1:10~50
IHC-P:~~1:10~50
Subtitle
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Cellular Location
Cell membrane; Peripheral membrane protein.
Tissue Location
Expressed in prostate, ovary, testis, placenta and prostate cancer cell lines. Localizes to basal and terminal prostate epithelial cells.
Antigen Region
438-468 aa
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(All lanes : Anti-POTE Antibody (C-term L446) at 1:500 dilutionLane 1: human ovary lysateLane 2: human testis lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-POTE Antibody (C-term L446) at 1:500 dilutionLane 1: human ovary lysateLane 2: human testis lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)-Paraffin
(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with POTE antibody (C-term L446), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)-Paraffin
(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with POTE antibody (C-term L446), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Related Product Information for anti-POTED antibody
POTE, is a highly homologous gene family located on numerous chromosomes and expressed in prostate, ovary, testis, placenta, and prostate cancer. The POTE protein contains seven ankyrin repeats between amino acids 140 and 380. Expression of POTE in prostate cancer and its undetectable expression in normal essential tissues make POTE a candidate for the immunotherapy of prostate cancer. The existence of a large number of closely related but rapidly diverging members, their location on multiple chromosomes and their limited expression pattern suggest an important role for the POTE gene family in reproductive processes.
Product Categories/Family for anti-POTED antibody
References
Bera T.K., Proc. Natl. Acad. Sci. U.S.A. 99:16975-16980 (2002). Bera,T.K., Gene 337, 45-53 (2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66394 Da
NCBI Official Full Name
uncharacterized protein LOC100288966
NCBI Official Symbol
LOC100288966
NCBI Protein Information
uncharacterized protein LOC100288966
UniProt Protein Name
POTE ankyrin domain family member D
Protein Family
UniProt Gene Name
POTED
UniProt Synonym Gene Names
A26B3; ANKRD21; POTE; Protein POTE
UniProt Entry Name
POTED_HUMAN
Uniprot Description
ANKRD21: a peripheral membrane protein expressed in prostate, ovary, testis, placenta and prostate cancer cell lines.
Protein type: Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: 21q11.2
Cellular Component: plasma membrane
Similar Products
Product Notes
The POTED poted (Catalog #AAA9217085) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POTE Antibody (C-term L446) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's POTE can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA). WB:~~1:500 IHC-P:~~1:10~50. Researchers should empirically determine the suitability of the POTED poted for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POTE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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