Rabbit anti-Human NMNAT1 Polyclonal Antibody | anti-NMNAT1 antibody

NMNAT1 Antibody (C-Term)

Cat. Num. AAA9216989

• Gene Names: NMNAT1; LCA9; NMNAT; PNAT1
• Reactivity: Human
• Applications: Western Blot
• Synonyms: NMNAT1; Polyclonal Antibody; NMNAT1 Antibody (C-Term); Purified Rabbit Polyclonal Antibody (Pab); Nicotinamide mononucleotide adenylyltransferase 1; NMN adenylyltransferase 1; 2.7.7.1; Nicotinate-nucleotide adenylyltransferase 1; NaMN adenylyltransferase 1; 2.7.7.18; NMNAT; anti-NMNAT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Sequence Length: 279

• Applicable Applications for anti-NMNAT1 antibody: Western Blot (WB)
• Application Notes: WB~~1:2000
• Antigen Type: Synthesized Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-NMNAT1 Antibody (C-Term) at 1:2000 dilution + human heart lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 32 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Anti-NMNAT1 Antibody (C-Term) at 1:2000 dilution + human brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 32 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-NMNAT1 antibody

Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, prefers NAD(+) and NAAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). Protects against axonal degeneration following mechanical or toxic insults.

Product Categories/Family for anti-NMNAT1 antibody

Metabolism; Neuroscience; Signal Transduction

References

Schweiger M.,et al.FEBS Lett. 492:95-100(2001). Emanuelli M.,et al.J. Biol. Chem. 276:406-412(2001). Fernando F.S.,et al.Gene 284:23-29(2002). Ota T.,et al.Nat. Genet. 36:40-45(2004). Gregory S.G.,et al.Nature 441:315-321(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 663071195
• NCBI GeneID: 64802
• NCBI Accession #: NP_001284707.1
• NCBI GenBank Nucleotide #: NM_001297778.1
• UniProt Accession #: Q9HAN9; Q8TAE9; Q9H247; Q9H6B6; B1AN63
• Molecular Weight: 31,932 Da
• NCBI Official Full Name: nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1 isoform 1
• NCBI Official Synonym Full Names: nicotinamide nucleotide adenylyltransferase 1
• NCBI Official Symbol: NMNAT1
• NCBI Official Synonym Symbols: LCA9; NMNAT; PNAT1
• NCBI Protein Information: nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1
• UniProt Protein Name: Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1
• UniProt Gene Name: NMNAT1
• UniProt Synonym Gene Names: NMNAT; NMN/NaMN adenylyltransferase 1; NMN adenylyltransferase 1; NaMN adenylyltransferase 1
• UniProt Entry Name: NMNA1_HUMAN

NCBI Description

This gene encodes an enzyme which catalyzes a key step in the biosynthesis of nicotinamide adenine dinucleotide (NAD). The encoded enzyme is one of several nicotinamide nucleotide adenylyltransferases, and is specifically localized to the cell nucleus. Activity of this protein leads to the activation of a nuclear deacetylase that functions in the protection of damaged neurons. Mutations in this gene have been associated with Leber congenital amaurosis 9. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are located on chromosomes 1, 3, 4, 14, and 15. [provided by RefSeq, Jul 2014]

Uniprot Description

Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP (PubMed:17402747). Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency (PubMed:17402747). Can use triazofurin monophosphate (TrMP) as substrate (PubMed:17402747). Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+ (PubMed:17402747). For the pyrophosphorolytic activity, prefers NAD+ and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively (PubMed:17402747). Involved in the synthesis of ATP in the nucleus, together with PARP1, PARG and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257). Fails to cleave phosphorylated dinucleotides NADP+, NADPH and NaADP+ (PubMed:17402747). Protects against axonal degeneration following mechanical or toxic insults ().