Rabbit RAD21 Polyclonal Antibody | anti-RAD21 antibody

RAD21 Antibody (C-Term)

Cat. Num. AAA9217312

• Gene Names: RAD21; HR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Bovine
• Applications: Western Blot, ELISA
• Synonyms: RAD21; Polyclonal Antibody; RAD21 Antibody (C-Term); Double-strand-break repair protein rad21 homolog; hHR21; Nuclear matrix protein 1; NXP-1; SCC1 homolog; HR21; KIAA0078; NXP1; anti-RAD21 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Bovine
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This RAD21 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 501-535 amino acids from human RAD21.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 631

• Applicable Applications for anti-RAD21 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB:~~1:2000
• Subtitle: Purified Rabbit Polyclonal Antibody (Pab)
• Cellular Location: Nucleus. Chromosome. Chromosome, centromere. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis.
• Antigen Type: Synthesized Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-RAD21 Antibody (C-Term) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: 293 whole cell lysateLane 3: A431 whole cell lysateLane 4: C2C12 whole cell lysateLane 5: C6 whole cell lysateLane 6: H-4-II-E whole cell lysateLane 7: MOLT-4 whole cell lysateLane 8: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 72 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-RAD21 antibody

Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.

Product Categories/Family for anti-RAD21 antibody

Primary Antibodies; Cancer; Cell Biology

References

McKay M.J.,et al.Genomics 36:305-315 (1996). Sadano H.,et al.Biochem. Biophys. Res. Commun. 267:418-422 (2000). Nomura N.,et al.DNA Res. 1:223-229 (1994). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 5453994
• NCBI GeneID: 5885
• NCBI Accession #: NP_006256.1
• NCBI GenBank Nucleotide #: NM_006265.2
• UniProt Accession #: O60216; Q15001; Q99568; A8K0E0
• Molecular Weight: 71690 Da
• NCBI Official Full Name: double-strand-break repair protein rad21 homolog
• NCBI Official Synonym Full Names: RAD21 cohesin complex component
• NCBI Official Symbol: RAD21
• NCBI Official Synonym Symbols: HR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21
• NCBI Protein Information: double-strand-break repair protein rad21 homolog
• UniProt Protein Name: Double-strand-break repair protein rad21 homolog
• Protein Family: Cofilin
• UniProt Gene Name: RAD21
• UniProt Synonym Gene Names: HR21; KIAA0078; NXP1; hHR21; NXP-1
• UniProt Entry Name: RAD21_HUMAN

NCBI Description

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008]

Uniprot Description

RAD21: Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. Found in a complex with SMC1A, SMC3, CDCA5, PDS5A/APRIN and PDS5B/SCC-112. Interacts with PDS5B and WAPAL; the interaction is direct. Belongs to the rad21 family.

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: chromatin; chromosome; chromosome, pericentric region; cohesin complex; cytosol; membrane; nucleoplasm

Molecular Function: protein binding

Biological Process: apoptosis; cell division; DNA recombination; double-strand break repair; meiotic recombination; mitosis; positive regulation of transcription from RNA polymerase II promoter; protein sumoylation; regulation of transcription from RNA polymerase II promoter; sister chromatid cohesion; transcription from RNA polymerase II promoter

Disease: Cornelia De Lange Syndrome 4