Rabbit anti-Human S100A9 Polyclonal Antibody | anti-S100A9 antibody

S100A9 Antibody (Center)

Cat. Num. AAA9215772

• Gene Names: S100A9; MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
• Reactivity: Human
• Applications: Western Blot
• Synonyms: S100A9; Polyclonal Antibody; S100A9 Antibody (Center); Purified Rabbit Polyclonal Antibody (Pab); Protein S100-A9; Calgranulin-B; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain; Migration inhibitory factor-related protein 14; MRP-14; p14; S100 calcium-binding protein A9; CAGB; CFAG; MRP14; anti-S100A9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This S100A9 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 22-56 amino acids from the Central region of human S100A9.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 114

• Applicable Applications for anti-S100A9 antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:500-1:1000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-S100A9 Antibody (Center) at 1:500-1:1000 dilutionLane 1: human breast lysateLane 2: human heart lysateLane 3: human lung lysateLane 4: human spleen lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 13 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-S100A9 antibody

S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH- oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF- kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread.

Product Categories/Family for anti-S100A9 antibody

Cancer; Cell Biology; Immunology; Signal Transduction

References

Odink K.,et al.Nature 330:80-82(1987). Lagasse E.,et al.Mol. Cell. Biol. 8:2402-2410(1988). Murao S.,et al.J. Biol. Chem. 264:8356-8360(1989). Wang M.,et al.Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases. Halleck A.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 4506773
• NCBI GeneID: 6280
• NCBI Accession #: NP_002956.1
• NCBI GenBank Nucleotide #: NM_002965.3
• UniProt Accession #: P06702; Q6FGA1; Q9NYM0; Q9UCJ1; D3DV36
• Molecular Weight: 13242
• NCBI Official Full Name: protein S100-A9
• NCBI Official Synonym Full Names: S100 calcium binding protein A9
• NCBI Official Symbol: S100A9
• NCBI Official Synonym Symbols: MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
• NCBI Protein Information: protein S100-A9
• UniProt Protein Name: Protein S100-A9
• Protein Family: Protein
• UniProt Gene Name: S100A9
• UniProt Synonym Gene Names: CAGB; CFAG; MRP14; MRP-14; p14
• UniProt Entry Name: S10A9_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. This antimicrobial protein exhibits antifungal and antibacterial activity. [provided by RefSeq, Nov 2014]

Uniprot Description

S100A9: a calcium-binding regulatory protein of the S-100 family expressed by macrophages in acutely inflammated tissues and in chronic inflammations. May be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells. Interacts with CEACAM3 in a calcium-dependent manner.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; cytoskeleton; extracellular region; plasma membrane; cytosol; nucleus

Molecular Function: arachidonic acid binding; antioxidant activity; signal transducer activity; RAGE receptor binding; protein binding; zinc ion binding; microtubule binding; calcium ion binding

Biological Process: chemokine production; positive regulation of blood coagulation; positive regulation of peptide secretion; response to lipopolysaccharide; signal transduction; activation of NF-kappaB transcription factor; regulation of translation; sequestering of zinc ion; cell-cell signaling; inflammatory response; caspase activation; neutrophil chemotaxis; chronic inflammatory response; cytokine production; positive regulation of cell growth; leukocyte migration during inflammatory response; response to ethanol; response to zinc ion; actin cytoskeleton reorganization; defense response to bacterium; autophagy; innate immune response; regulation of integrin biosynthetic process; defense response to fungus; positive regulation of inflammatory response; regulation of cytoskeleton organization and biogenesis