Western Blot (WB)
(Western blot analysis of extracts from MOLT4, using SDCCAG3 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit anti-Human, Rat SDCCAG3 Polyclonal Antibody | anti-SDCCAG3 antibody
SDCCAG3 Antibody
Gene Names
ENTR1; SDDAG3; NY-CO-3; SDCCAG3
Reactivity
Human, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
SDCCAG3; Polyclonal Antibody; SDCCAG3 Antibody; Antigen NY CO 3; NY CO 3; NY-CO-3; OTTHUMP00000022582; SDCCAG 3; Serologically defined colon cancer antigen 3; anti-SDCCAG3 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SDCCAG3 Antibody detects endogenous levels of total SDCCAG3.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
435
Applicable Applications for anti-SDCCAG3 antibody
ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human SDCCAG3, corresponding to a region within N-terminal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from MOLT4, using SDCCAG3 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from MOLT4, using SDCCAG3 Antibody. The lane on the left was treated with blocking peptide.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612435 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612435 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612435 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612435 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Related Product Information for anti-SDCCAG3 antibody
Function: Endosome-associated protein that plays a role in membrane receptor sorting, cytokinesis and ciliogenesis (PubMed:23108400, PubMed:25278552, PubMed:27767179). Involved in the endosome-to-plasma membrane trafficking and recycling of SNX27-retromer-dependent cargo proteins, such as GLUT1 (PubMed:25278552). Involved in the regulation of cytokinesis; the function may involve PTPN13 and GIT1 (PubMed:23108400). Plays a role in the formation of cilia (PubMed:27767179). Involved in cargo protein localization, such as PKD2, at primary cilia (PubMed:27767179). Involved in the presentation of the tumor necrosis factor (TNF) receptor TNFRSF1A on the cell surface, and hence in the modulation of the TNF-induced apoptosis (By similarity).
Post Translational Modifications: Phosphorylated.
Subcellular Location: Cytoplasm. Early endosome. Endosome. Recycling endosome. Midbody. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Cilium basal body. Note: Colocalizes in a WASHC2-dependent manner with the retromer CSC complex at endosomes (PubMed:25278552). During cytokinesis colocalized with PTPN13 at the midbody (PubMed:23108400). Colocalizes with IFT88 and gamma-tubulin at the basal body of primary cilia (PubMed:27767179). Colocalizes with IFT88 and pericentrin at the centrosome (PubMed:27767179).
Tissue Specificity: Expressed in the colon (at protein level).
Subunit Structure: Found in a complex with ENTR1, PTPN13 and GIT1 (PubMed:23108400). Interacts with PTPN13 (via the FERM domain) (PubMed:23108400). Interacts (via N-terminus) with GIT1 (via N- and C-terminus); this interaction is direct (PubMed:23108400). Interacts with NOD2 (PubMed:27812135). Interacts (via N-terminus) with IFT88 (PubMed:27767179). Interacts with VPS35 (PubMed:25278552).
Similarity: Tne N-terminal domain is necessary and sufficient for basal body localization and ciliogenesis.Belongs to the ENTR1 family.
Post Translational Modifications: Phosphorylated.
Subcellular Location: Cytoplasm. Early endosome. Endosome. Recycling endosome. Midbody. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Cilium basal body. Note: Colocalizes in a WASHC2-dependent manner with the retromer CSC complex at endosomes (PubMed:25278552). During cytokinesis colocalized with PTPN13 at the midbody (PubMed:23108400). Colocalizes with IFT88 and gamma-tubulin at the basal body of primary cilia (PubMed:27767179). Colocalizes with IFT88 and pericentrin at the centrosome (PubMed:27767179).
Tissue Specificity: Expressed in the colon (at protein level).
Subunit Structure: Found in a complex with ENTR1, PTPN13 and GIT1 (PubMed:23108400). Interacts with PTPN13 (via the FERM domain) (PubMed:23108400). Interacts (via N-terminus) with GIT1 (via N- and C-terminus); this interaction is direct (PubMed:23108400). Interacts with NOD2 (PubMed:27812135). Interacts (via N-terminus) with IFT88 (PubMed:27767179). Interacts with VPS35 (PubMed:25278552).
Similarity: Tne N-terminal domain is necessary and sufficient for basal body localization and ciliogenesis.Belongs to the ENTR1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)47kD.
Predicted Molecular Weight: (Calculated)48kDa.
Predicted Molecular Weight: (Calculated)48kDa.
NCBI Official Full Name
Endosome-associated-trafficking regulator 1
NCBI Official Synonym Full Names
endosome associated trafficking regulator 1
NCBI Official Symbol
ENTR1
NCBI Official Synonym Symbols
SDDAG3; NY-CO-3; SDCCAG3
NCBI Protein Information
endosome-associated-trafficking regulator 1
UniProt Protein Name
Serologically defined colon cancer antigen 3
Protein Family
UniProt Gene Name
SDCCAG3
UniProt Entry Name
SDCG3_HUMAN
Uniprot Description
SDCCAG3: May be involved in modulation of TNF response. May be involved in presentation of TNFRSF1A on the cell surface. Belongs to the SDCCAG3 family. 4 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 9q34.3
Cellular Component: recycling endosome; retromer complex; early endosome; midbody; endosome
Molecular Function: protein binding
Biological Process: protein transport; cell division; cell cycle; regulation of cytokinesis
Research Articles on SDCCAG3
Similar Products
Product Notes
The SDCCAG3 sdccag3 (Catalog #AAA9612435) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SDCCAG3 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SDCCAG3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the SDCCAG3 sdccag3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SDCCAG3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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