Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western blot analysis of RPA43 Antibody (C-term) in Jurkat,K562 cell line lysates (35ug/lane).RPA43 (arrow) was detected using the purified Pab.)

Rabbit anti-Human RPA43 Polyclonal Antibody | anti-TWISTNB antibody

RPA43 Antibody (C-term)

Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RPA43; Polyclonal Antibody; RPA43 Antibody (C-term); DNA-directed RNA polymerase I subunit RPA43; Twist neighbor protein; TWISTNB; anti-TWISTNB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RPA43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-326 amino acids from the C-terminal region of human RPA43.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
298-326
Sequence Length
338
Applicable Applications for anti-TWISTNB antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of RPA43 Antibody (C-term) in Jurkat,K562 cell line lysates (35ug/lane).RPA43 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of RPA43 Antibody (C-term) in Jurkat,K562 cell line lysates (35ug/lane).RPA43 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(RPA43 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPA43 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (RPA43 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPA43 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-TWISTNB antibody
RPA43 is DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is component of RNA polymerase I which synthesizes ribosomal RNA precursors. Through its association with RRN3/TIF-IA may be involved in recruitment of Pol I to rDNA promoters.
References
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Yuan, X., et al. EMBO Rep. 3(11):1082-1087(2002)
Kosan, C., et al. Cytogenet. Genome Res. 97 (3-4), 167-170 (2002)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37432
NCBI Official Full Name
DNA-directed RNA polymerase I subunit RPA43
NCBI Official Synonym Full Names
TWIST neighbor
NCBI Official Symbol
TWISTNB
NCBI Protein Information
DNA-directed RNA polymerase I subunit RPA43
UniProt Protein Name
DNA-directed RNA polymerase I subunit RPA43
UniProt Gene Name
TWISTNB
UniProt Entry Name
RPA43_HUMAN

Uniprot Description

TWISTNB: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Through its association with RRN3/TIF-IA may be involved in recruitment of Pol I to rDNA promoters. Belongs to the eukaryotic RPA43 RNA polymerase subunit family.

Protein type: Nucleolus

Chromosomal Location of Human Ortholog: 7p21.1

Cellular Component: microtubule cytoskeleton; nucleoplasm; nucleolus; nucleus; DNA-directed RNA polymerase I complex

Biological Process: negative regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; gene expression; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter; regulation of gene expression, epigenetic

Research Articles on TWISTNB

Similar Products

Product Notes

The TWISTNB twistnb (Catalog #AAA9214729) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 298-326. The RPA43 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RPA43 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the TWISTNB twistnb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPA43, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.