Rabbit anti-Zebrafish DANRE tcf21 Polyclonal Antibody | anti-TCF21 antibody

DANRE tcf21 Antibody (N-term)

Cat. Num. AAA9213778

• Gene Names: tcf21; zgc:123313
• Reactivity: Zebrafish
• Applications: Western Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: DANRE tcf21; Polyclonal Antibody; DANRE tcf21 Antibody (N-term); Transcription factor 21 {ECO:0000312|EMBL:AAI079691}; TCF-21; Capsulin {ECO:0000312|EMBL:ABU954061}; Epicardin; MyoRa2; TCF21; anti-TCF21 antibody

• Host: Rabbit
• Reactivity: Zebrafish
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This DANRE tcf21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-35 amino acids from the N-terminal region of DANRE tcf21.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 5-35
• Sequence Length: 176

• Applicable Applications for anti-TCF21 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Crown Antibody: Yes
• Antigen Source: Zebrafish
• Cellular Location: Nucleus {ECO:0000250|UniProtKB:O43680, ECO:0000255|PROSITE-ProRule:PRU00981}
• Tissue Location: Expressed in the cranial paraxial mesoderm from 20 hpf and subsequently becomes restricted to the pharyngeal mesoderm that will form the muscle. Expression in the proepicardial organ is first seen at 40hpf in a cluster of cells between the myocardium and yolk Also expressed in the developing arches. Expression begins to surround the heart by day 3 of development, and by 96 hpf, expression is restricted to the outer epicardial layer surrounding the myocardium EMBL; EF632300; ABU95406.1; -; mRNA EMBL; BC107968; AAI07969.1; -; mRNA RefSeq; NP_001032770.1; NM_001037681.1 SMR; Q32PV5; - STRING; 7955.ENSDARP00000053538; - PaxDb; Q32PV5; - GeneID; 558148; - KEGG; dre:558148; - CTD; 6943; - ZFIN; ZDB-GENE-051113-88; tcf21 eggNOG; KOG4029; Eukaryota eggNOG; ENOG411227D; LUCA InParanoid; Q32PV5; - OrthoDB; 1374128at2759; - PhylomeDB; Q32PV5; - PRO; PR:Q32PV5; - Proteomes; UP000000437; Unplaced GO; GO:0005634; C:nucleus; IDA:UniProtKB GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISS:UniProtKB GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISS:UniProtKB GO; GO:0070888; F:E-box binding; ISS:UniProtKB GO; GO:0046983; F:protein dimerization activity; ISS:UniProtKB GO; GO:0000977; F:RNA polymerase II regulatory region sequence-specific DNA binding; IBA:GO_Central GO; GO:0060840; P:artery development; IGI:ZFIN GO; GO:0001658; P:branching involved in ureteric bud morphogenesis; ISS:UniProtKB GO; GO:0014707; P:branchiomeric skeletal muscle development; IMP:ZFIN GO; GO:0060435; P:bronchiole development; ISS:UniProtKB GO; GO:0060539; P:diaphragm development; ISS:UniProtKB GO; GO:0048557; P:embryonic digestive tract morphogenesis; ISS:UniProtKB GO; GO:0030855; P:epithelial cell differentiation; ISS:UniProtKB GO; GO:0048732; P:gland development; ISS:UniProtKB GO; GO:0032835; P:glomerulus development; ISS:UniProtKB GO; GO:0001822; P:kidney development; ISS:UniProtKB GO; GO:0048286; P:lung alveolus development; ISS:UniProtKB GO; GO:0060425; P:lungmorphogenesis; ISS:UniProtKB GO; GO:0060426; P:lung vasculature development; ISS:UniProtKB GO; GO:0072277; P:metanephric glomerular capillary formation; ISS:UniProtKB GO; GO:0072162; P:metanephric mesenchymal
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(DANRE tcf21 Antibody (N-term) western blot analysis in zebra fish brain tissue lysates (35ug/lane).This demonstrates the DANRE tcf21 antibody detected the DANRE tcf21 protein (arrow).)

Related Product Information for anti-TCF21 antibody

Involved in epithelial-mesenchymal interactions in kidney and lung morphogenesis that include epithelial differentiation and branching morphogenesis (By similarity).

Product Categories/Family for anti-TCF21 antibody

Crown Antibodies; Cardiovascular; Cell Biology

References

Wang Y., et al. J. Mol. Evol. 68:629-640(2009).
Chang M.-Y., et al. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
Serluca F.C., et al. Dev. Biol. 315:18-27(2008).
Knight R.D., et al. J. Exp. Zool. B Mol. Dev. Evol. 310:355-369(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 83415108
• NCBI GeneID: 558148
• NCBI Accession #: NP_001032770.1
• NCBI GenBank Nucleotide #: NM_001037681.1
• UniProt Accession #: Q32PV5
• Molecular Weight: 19346
• NCBI Official Full Name: transcription factor 21
• NCBI Official Synonym Full Names: transcription factor 21
• NCBI Official Symbol: tcf21
• NCBI Official Synonym Symbols: zgc:123313
• NCBI Protein Information: transcription factor 21
• UniProt Protein Name: Transcription factor 21
• Protein Family: Transcription factor
• UniProt Gene Name: tcf21
• UniProt Synonym Gene Names: TCF-21
• UniProt Entry Name: TCF21_DANRE

Uniprot Description

Involved in epithelial-mesenchymal interactions in kidney and lung morphogenesis that include epithelial differentiation and branching morphogenesis.