Rabbit anti-Human GNAI3 Polyclonal Antibody | anti-GNAI3 antibody

GNAI3 Antibody

Cat. Num. AAA9213565

• Gene Names: GNAI3; 87U6; ARCND1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: GNAI3; Polyclonal Antibody; GNAI3 Antibody; Guanine nucleotide-binding protein G(k) subunit alpha; G(i) alpha-3; anti-GNAI3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This GNAI3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 309-343 amino acids from the human region of human GNAI3.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 354

• Applicable Applications for anti-GNAI3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:2000
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-GNAI3 Antibodyat 1:2000 dilution + HT-29 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 41 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-GNAI3 antibody

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor- regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.

References

Didsbury J.R.,et al.FEBS Lett. 219:259-263(1987).
Beals C.R.,et al.Proc. Natl. Acad. Sci. U.S.A. 84:7886-7890(1987).
Itoh H.,et al.J. Biol. Chem. 263:6656-6664(1988).
Codina J.,et al.J. Biol. Chem. 263:6746-6750(1988).
Kim S.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:4153-4157(1988).

NCBI and Uniprot Product Information

• NCBI GI #: 5729850
• NCBI GeneID: 2773
• NCBI Accession #: NP_006487.1
• NCBI GenBank Nucleotide #: NM_006496.3
• UniProt Accession #: P08754; P17539; Q5TZX1
• Molecular Weight: 40532
• NCBI Official Full Name: guanine nucleotide-binding protein G(k) subunit alpha
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3
• NCBI Official Symbol: GNAI3
• NCBI Official Synonym Symbols: 87U6; ARCND1
• NCBI Protein Information: guanine nucleotide-binding protein G(k) subunit alpha
• UniProt Protein Name: Guanine nucleotide-binding protein G(k) subunit alpha
• UniProt Gene Name: GNAI3
• UniProt Entry Name: GNAI3_HUMAN

NCBI Description

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway. [provided by RefSeq, Jun 2012]

Uniprot Description

G-alpha i3: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor- regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. Defects in GNAI3 are the cause of auriculocondylar syndrome 1 (ARCND1). ARCND1 is an autosomal dominant craniofacial malformation syndrome characterized by variable mandibular anomalies, including mild to severe micrognathia, temporomandibular joint ankylosis, cleft palate, and a characteristic ear malformation that consists of separation of the lobule from the external ear, giving the appearance of a question mark (question-mark ear). Other frequently described features include prominent cheeks, cupped and posteriorly rotated ears, preauricular tags, and microstomia. Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Protein type: G protein, heterotrimeric; G protein, heterotrimeric alpha G((i/o/t/z)); G protein

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: Golgi apparatus; centrosome; membrane; lysosomal membrane; cytoplasm; plasma membrane; heterotrimeric G-protein complex; midbody; lipid raft

Molecular Function: GTPase activity; protein domain specific binding; signal transducer activity; GTP binding; metal ion binding; G-protein beta/gamma-subunit binding; GTPase activating protein binding; metabotropic serotonin receptor binding

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; vesicle fusion; platelet activation; synaptic transmission; transport; cell division; metabolic process; negative regulation of adenylate cyclase activity; G-protein signaling, adenylate cyclase inhibiting pathway; blood coagulation; cell cycle

Disease: Auriculocondylar Syndrome 1