GNAI3 recombinant protein

GNAI3, 1-354aa, Human, His tag, E Coli

Cat. Num. AAA204545

• Gene Names: GNAI3; 87U6; ARCND1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: GNAI3; 1-354aa; Human; His tag; E Coli; Guanine nucleotide-binding protein G (k) subunit alpha; 87u6; GNAI3 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100mM NaCl
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGCTLSA EDKAAVERSK MIDRNLREDG EKAAKEVKLL LLGAGESGKS TIVKQMKIIH EDGYSEDECK QYKVVVYSNT IQSIIAIIRA MGRLKIDFGE AARADDARQL FVLAGSAEEG VMTPELAGVI KRLWRDGGVQ ACFSRSREYQ LNDSASYYLN DLDRISQSNY IPTQQDVLRT RVKTTGIVET HFTFKDLYFK MFDVGGQRSE RKKWIHCFEG VTAIIFCVAL SDYDLVLAED EEMNRMHESM KLFDSICNNK WFTETSIILF LNKKDLFEEK IKRSPLTICY PEYTGSNTYE EAAAYIQCQF EDLNRRKDTK EIYTHFTCAT DTKNVQFVFD AVTDVIIKNN LKECGLY
• Sequence Length: 354

• Applicable Applications for GNAI3 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GNAI3 recombinant protein

GNAI3 belongs to the G-alpha family and G (i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G (k) is the stimulatory G protein of receptor-regulated K+ channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. This protein plays a role in cell division. GNAI3 has been shown to interact with RGS14, RIC8A, RGS18, S1PR1, RGS12, RGS16, RGS19, RGS10 and RGS5. Recombinant human GNAI3 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for GNAI3 recombinant protein

Signal Transduction

References

Cho H., et al. (2007) J. Cell Biol. 178:245-255; Jones, D.T., et al. (1990) J. Biol. Chem. 265: 2671-2676.

NCBI and Uniprot Product Information

• NCBI GI #: 5729850
• NCBI GeneID: 2773
• NCBI Accession #: NP_006487
• NCBI GenBank Nucleotide #: NM_006496.3
• UniProt Accession #: P08754; P17539; Q5TZX1
• Molecular Weight: 43.0kDa (377aa), confirmed by MALDI-TOF
• NCBI Official Full Name: guanine nucleotide-binding protein G(k) subunit alpha
• NCBI Official Synonym Full Names: G protein subunit alpha i3
• NCBI Official Symbol: GNAI3
• NCBI Official Synonym Symbols: 87U6; ARCND1
• NCBI Protein Information: guanine nucleotide-binding protein G(k) subunit alpha
• UniProt Protein Name: Guanine nucleotide-binding protein G(k) subunit alpha
• UniProt Gene Name: GNAI3

NCBI Description

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway. [provided by RefSeq, Jun 2012]

Uniprot Description

Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:8774883, PubMed:18434541, PubMed:19478087). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed:19478087). Stimulates the activity of receptor-regulated K+ channels (PubMed:2535845). The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (PubMed:17635935).