Western Blot (WB)
(Western blot analysis of DCL-1 Antibody (Center) in mouse liver tissue lysates (35ug/lane). DCL-1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, mouse DCL-1 Polyclonal Antibody | anti-CD302 antibody
DCL-1 Antibody (Center)
Gene Names
CD302; DCL1; DCL-1; BIMLEC; CLEC13A
Reactivity
Human, mouse
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DCL-1; Polyclonal Antibody; DCL-1 Antibody (Center); CD302 antigen; C-type lectin BIMLEC; C-type lectin domain family 13 member A; DEC205-associated C-type lectin 1; Type I transmembrane C-type lectin receptor DCL-1; CD302; CLEC13A; DCL1; KIAA0022; anti-CD302 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DCL-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-170 amino acids from the Central region of human DCL-1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.466 (varies by lot)
Sequence Positions
143-170
Sequence Length
1,873
Applicable Applications for anti-CD302 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IHC-P~~1:50~100
FC~~1:10~50
Antigen Type
Synthetic Peptide
Clone Names
RB19594
Antigen Source
Human
Function
Potential multifunctional C-type lectin receptor that may play roles in endocytosis and phagocytosis as well as in cell adhesion and migration.
Cellular Location
Membrane; Single-pass type I membrane protein. Cell projection, filopodium. Cytoplasm, cell cortex. Cell projection, microvillus. Note=Colocalizes with F-actin in filopodia, cellular cortex and microvilli of the apical cell surface
Tissue Location
Expressed at moderate levels in monocytes, myeloid blood dendritic cells and granulocytes and at low levels in plasmacytoid blood dendritic cells, monocyte-derived ma crophages and monocyte-derived dendritic cells, with no expression detected in T-lymphocytes, B-lymphocytes and natural killer cells (at protein level). Expressed widely in different tissues, with highest expression levels in liver, lung, peripheral blood leukocytes and spleen, and lowest levels in neuronal tissues, skeletal muscle and ovary. Isoform 2 and isoform 3 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed- Sternberg (HRS) cells.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of DCL-1 Antibody (Center) in mouse liver tissue lysates (35ug/lane). DCL-1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of DCL-1 Antibody (Center) in mouse liver tissue lysates (35ug/lane). DCL-1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma with DCL-1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma with DCL-1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(DCL-1 Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(DCL-1 Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-CD302 antibody
CD302 is a C-type lectin receptor involved in cell adhesion and migration, as well as endocytosis and phagocytosis.
References
Kato,M., J. Immunol. 179 (9), 6052-6063 (2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26183
NCBI Official Full Name
CD302 antigen isoform 2
NCBI Official Synonym Full Names
CD302 molecule
NCBI Official Symbol
CD302
NCBI Official Synonym Symbols
DCL1; DCL-1; BIMLEC; CLEC13A
NCBI Protein Information
CD302 antigen
UniProt Protein Name
CD302 antigen
Protein Family
UniProt Gene Name
CD302
UniProt Synonym Gene Names
CLEC13A; DCL1; KIAA0022
UniProt Entry Name
CD302_HUMAN
NCBI Description
CD302 is a C-type lectin receptor involved in cell adhesion and migration, as well as endocytosis and phagocytosis (Kato et al., 2007 [PubMed 17947679]).[supplied by OMIM, Aug 2008]
Uniprot Description
CD302: Potential multifunctional C-type lectin receptor that may play roles in endocytosis and phagocytosis as well as in cell adhesion and migration. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 2q24.2
Cellular Component: microvillus; membrane; integral to membrane; cell cortex; filopodium
Molecular Function: carbohydrate binding
Biological Process: phagocytosis
Research Articles on CD302
Similar Products
Product Notes
The CD302 cd302 (Catalog #AAA9212379) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 143-170. The DCL-1 Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DCL-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50. Researchers should empirically determine the suitability of the CD302 cd302 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DCL-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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