Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit anti-Human MLLT6 Polyclonal Antibody | anti-MLLT6 antibody
MLLT6 Antibody (C-term)
Gene Names
MLLT6; AF17
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MLLT6; Polyclonal Antibody; MLLT6 Antibody (C-term); Protein AF-17; ALL1-fused gene from chromosome 17 protein; AF17; anti-MLLT6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MLLT6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1056-1086 amino acids from the C-terminal region of human MLLT6.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
1056-1086
Sequence Length
1093
Applicable Applications for anti-MLLT6 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC~~1:50~100
WB~~1:1000
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB)
(Western blot analysis of MLLT6 (arrow) using rabbit polyclonal MLLT6 Antibody (E1071). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MLLT6 gene.)
Western Blot (WB)
(Western blot analysis of MLLT6 (arrow) using rabbit polyclonal MLLT6 Antibody (E1071). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MLLT6 gene.)
Related Product Information for anti-MLLT6 antibody
Band 11q23 chromosome locations are associated with approximately 10% of patients with acute lymphoblastic leukemia (ALL) and more than 5% of patients with acute myeloid leukemia (AML). 11q23 translocation associated leukemias typically jointly express lymphoid and myeloid markers and exhibit poor prognosis. The gene at 11q23 involved in the translocations is known by multiple names, including ALL1, HRX, MLL, and TRX1. One of the more infrequent translocations, t(11;17)(q23;q21), MLLT6, encodes a protein of 1,093 amino acids, containing a leucine-zipper dimerization motif 3-prime to the fusion point and a cysteine-rich domain at the end terminus that can be arranged in 3 zinc fingers. MMLT6 contains amino acid runs associated with domains involved in transcriptional repression or activation. It has been proposed that MLLT6 represses truncated ALL1 function or inhibits function of the normal protein in leukemic cells.
References
Prasad, R., et al., Proc. Natl. Acad. Sci. U.S.A. 91(17):8107-8111 (1994).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
112076
NCBI Official Full Name
protein AF-17
NCBI Official Synonym Full Names
myeloid/lymphoid or mixed-lineage leukemia; translocated to, 6
NCBI Official Symbol
MLLT6
NCBI Official Synonym Symbols
AF17
NCBI Protein Information
protein AF-17
UniProt Protein Name
Protein AF-17
Protein Family
UniProt Gene Name
MLLT6
UniProt Synonym Gene Names
AF17
UniProt Entry Name
AF17_HUMAN
Uniprot Description
MLLT6: A chromosomal aberration involving MLLT6 is associated with acute leukemias. Translocation t(11;17)(q23;q21) with MLL/HRX. The result is a rogue activator protein.
Protein type: Transcription factor; Oncoprotein
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: nucleus
Molecular Function: protein binding; zinc ion binding
Biological Process: regulation of transcription, DNA-dependent
Research Articles on MLLT6
Similar Products
Product Notes
The MLLT6 mllt6 (Catalog #AAA9211542) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1056-1086. The MLLT6 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MLLT6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). IHC~~1:50~100 WB~~1:1000. Researchers should empirically determine the suitability of the MLLT6 mllt6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLLT6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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