Western Blot (WB)
(Western blot analysis of CCT3 Antibody (Center) in MCF7,CEM,K562,HL-60,Hela cell line lysates (35ug/lane).CCT3 (arrow) was detected using the purified Pab.)
Rabbit CCT3 Polyclonal Antibody | anti-CCT3 antibody
CCT3 Antibody (Center)
Western Blot (WB)
(Western blot analysis of CCT3 Antibody (Center) in MCF7,CEM,K562,HL-60,Hela cell line lysates (35ug/lane).CCT3 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of CCT3 Antibody (Center) in MCF7,CEM,K562,HL-60,Hela cell line lysates (35ug/lane).CCT3 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of CCT3 (arrow) using rabbit polyclonal CCT3 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CCT3 gene (Lane 2).)
Western Blot (WB)
(Western blot analysis of CCT3 (arrow) using rabbit polyclonal CCT3 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CCT3 gene (Lane 2).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CCT3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CCT3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of K562 cells using CCT3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of K562 cells using CCT3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants have been characterized for this gene. In addition, a pseudogene of this gene has been found on chromosome 8. [provided by RefSeq, Aug 2010]
Uniprot Description
CCT-gamma: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Belongs to the TCP-1 chaperonin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytoskeletal; Chaperone
Chromosomal Location of Human Ortholog: 1q23
Cellular Component: chaperonin-containing T-complex; microtubule; cytoskeleton; cytoplasm; plasma membrane; cytosol
Molecular Function: protein binding; unfolded protein binding; ATP binding
Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; binding of sperm to zona pellucida; pore complex biogenesis
Research Articles on CCT3
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Product Notes
The CCT3 cct3 (Catalog #AAA9211362) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 298-326. The CCT3 Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's CCT3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the CCT3 cct3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCT3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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