Rabbit anti-Human DKK4 Polyclonal Antibody | anti-DKK4 antibody

DKK4 Antibody (C-term)

Cat. Num. AAA9210543

• Gene Names: DKK4; DKK-4
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: DKK4; Polyclonal Antibody; DKK4 Antibody (C-term); Dickkopf-related protein 4; Dickkopf-4; Dkk-4; hDkk-4; Dickkopf-related protein 4 short form; anti-DKK4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This DKK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-217 amino acids from the C-terminal region of human DKK4.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 188-217
• Sequence Length: 224

• Applicable Applications for anti-DKK4 antibody: ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(The anti-DKK4 C-term Pab is used in Western blot to detect DKK4 in A375 cell lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-DKK4 antibody

DKK4, like DKK1, DKK2, and DKK3, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T-cell, esophagus, and lung cDNA libraries. DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors.

Product Categories/Family for anti-DKK4 antibody

Signal Transduction

References

Krupnik, V.E., et al., Gene 238(2):301-313 (1999).
Yoshida, S., et al., Cytogenet. Cell Genet. 94 (1-2), 88-89 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 7657025
• NCBI GeneID: 27121
• NCBI Accession #: NP_055235.1
• NCBI GenBank Nucleotide #: NM_014420.2
• UniProt Accession #: Q9UBT3; Q3KNX0; Q9Y4C3
• Molecular Weight: 24876
• NCBI Official Full Name: dickkopf-related protein 4
• NCBI Official Synonym Full Names: dickkopf WNT signaling pathway inhibitor 4
• NCBI Official Symbol: DKK4
• NCBI Official Synonym Symbols: DKK-4
• NCBI Protein Information: dickkopf-related protein 4
• UniProt Protein Name: Dickkopf-related protein 4
• Protein Family: Dickkopf-related protein
• UniProt Gene Name: DKK4
• UniProt Synonym Gene Names: Dickkopf-4; Dkk-4; hDkk-4
• UniProt Entry Name: DKK4_HUMAN

NCBI Description

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2. [provided by RefSeq, Jul 2008]

Uniprot Description

DKK4: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Belongs to the dickkopf family.

Protein type: Inhibitor; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8p11.2-p11.1

Cellular Component: extracellular region

Biological Process: Wnt receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; multicellular organismal development