Western Blot (WB)
(IRF9 Antibody (N-term)(MBS9209363) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the IRF9 antibody detected the IRF9 protein (arrow).)
Rabbit anti-Human, Mouse IRF9 Polyclonal Antibody | anti-IRF9 antibody
IRF9 Antibody (N-term)
WB~~1:2000
IHC-P~~1:10~50
Western Blot (WB)
(IRF9 Antibody (N-term)(MBS9209363) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the IRF9 antibody detected the IRF9 protein (arrow).)
Western Blot (WB)
(IRF9 Antibody (N-term)(MBS9209363) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the IRF9 antibody detected the IRF9 protein (arrow).)
Western Blot (WB)
(Anti-IRF9 Antibody (N-term) at 1:2000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-IRF9 Antibody (N-term) at 1:2000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(IRF9 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IRF9 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(IRF9 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IRF9 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of IRF9 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of IRF9 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010)
Watanabe, T., et al. J. Clin. Invest. 120(5):1645-1662(2010)
Mosbruger, T.L., et al. J. Infect. Dis. 201(9):1371-1380(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
NCBI and Uniprot Product Information
Uniprot Description
IRF9: Transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. Belongs to the IRF family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus
Molecular Function: protein binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; cell surface receptor linked signal transduction; regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; interferon type I biosynthetic process; defense response to virus
Research Articles on IRF9
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Product Notes
The IRF9 irf9 (Catalog #AAA9209363) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 75-104. The IRF9 Antibody (N-term) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IRF9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Parffin (IHC-P), Immunofluorescence (IF). IF~~1:10~50 WB~~1:2000 IHC-P~~1:10~50. Researchers should empirically determine the suitability of the IRF9 irf9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRF9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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