Rabbit MyD88 Polyclonal Antibody | anti-MYD88 antibody

MyD88 Antibody (Center)

Cat. Num. AAA9209108

• Gene Names: MYD88; MYD88D
• Reactivity: Human, mouse (Predicted Reactivity: Monkey)
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: MyD88; Polyclonal Antibody; MyD88 Antibody (Center); Myeloid differentiation primary response protein MyD88; MYD88; anti-MYD88 antibody

• Host: Rabbit
• Reactivity: Human, mouse (Predicted Reactivity: Monkey)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This MyD88 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-164 amino acids from the Central region of human MyD88.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 136-164
• Sequence Length: 304

• Applicable Applications for anti-MYD88 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of MyD88 Antibody (Center) in mouse lung tissue lysates (35ug/lane). MyD88 (arrow) was detected using the purified Pab.(2ug/ml))

Western Blot (WB)

(Western blot analysis of MyD88 Antibody (Center) in HepG2 cell line lysates (35ug/lane). MyD88 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with MyD88 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(MyD88 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MYD88 antibody

Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. It acts via IRAK1, IRAK2 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response and increases IL-8 transcription. It may be involved in myeloid differentiation.

Product Categories/Family for anti-MYD88 antibody

Cardiovascular; Immunology; Signal Transduction

References

Bannon,C., et.al., Biochem. J. 423 (1), 119-128 (2009)
Burns,K., et.al., J. Biol. Chem. 273 (20), 12203-12209 (1998)

NCBI and Uniprot Product Information

• NCBI GI #: 289546500
• NCBI GeneID: 4615
• NCBI Accession #: NP_001166037.1; NP_001166039.1; NP_001166040.1; NP_002459.2
• NCBI GenBank Nucleotide #: NM_001172566.1
• UniProt Accession #: Q99836; P78397; Q53XS7; B4DKH8; B4DKU4; B4DQ60; B4DQ72; J3KPU4; J3KQ87; J3KQJ6
• Molecular Weight: 33233
• NCBI Official Full Name: myeloid differentiation primary response protein MyD88 isoform 5
• NCBI Official Synonym Full Names: myeloid differentiation primary response 88
• NCBI Official Symbol: MYD88
• NCBI Official Synonym Symbols: MYD88D
• NCBI Protein Information: myeloid differentiation primary response protein MyD88
• UniProt Protein Name: Myeloid differentiation primary response protein MyD88
• Protein Family: Myeloid differentiation primary response protein
• UniProt Gene Name: MYD88
• UniProt Entry Name: MYD88_HUMAN

NCBI Description

This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

Uniprot Description

MYD88: Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Homodimer. Also forms heterodimers with TIRAP. Binds to TLR2, TLR4, IRAK1, IRAK2 and IRAK4 via their respective TIR domains. Interacts with IL18R1. Interacts with BMX, IL1RL1 and IRF7. Interacts with LRRFIP1 and LRRFIP2; this interaction positively regulates Toll-like receptor (TLR) signaling in response to agonist. Interacts with FLII. LRRFIP1 and LRRFIP2 compete with FLII for MYD88-binding. Interacts with IRF1. Ubiquitous. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3p22

Cellular Component: plasma membrane; endosome membrane; cytosol

Molecular Function: identical protein binding; protein binding; death receptor binding

Biological Process: positive regulation of interleukin-17 production; positive regulation of I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; positive regulation of interleukin-23 production; positive regulation of interleukin-6 production; signal transduction; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; cell surface receptor linked signal transduction; regulation of inflammatory response; positive regulation of interferon type I production; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway; negative regulation of apoptosis

Disease: Macroglobulinemia, Waldenstrom, Susceptibility To, 1; Myd88 Deficiency