Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with TCF25 Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TCF25 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).TCF25 immunoreactivity is localized to Cytoplasm significantly. )
Rabbit anti-Human TCF25 Polyclonal Antibody | anti-TCF25 antibody
TCF25, NT (TCF25, KIAA1049, NULP1, Transcription factor 25, Nuclear localized protein 1) (APC)
Gene Names
TCF25; Hulp1; NULP1; FKSG26; PRO2620; hKIAA1049
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
TCF25; Polyclonal Antibody; NT (TCF25; KIAA1049; NULP1; Transcription factor 25; Nuclear localized protein 1) (APC); Nuclear localized protein 1; anti-TCF25 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-TCF25 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
TCF25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-215 amino acids from the N-terminal region of human TCF25.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with TCF25 Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TCF25 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).TCF25 immunoreactivity is localized to Cytoplasm significantly. )
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with TCF25 Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TCF25 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).TCF25 immunoreactivity is localized to Cytoplasm significantly. )
Western Blot (WB)
(TCF25 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the TCF25 antibody detected the TCF25 protein (arrow).)
Western Blot (WB)
(TCF25 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the TCF25 antibody detected the TCF25 protein (arrow).)
Related Product Information for anti-TCF25 antibody
TCF25 is a member of the basic helix-loop-helix (bHLH) family of transcription factors that are important in embryonic development (Steen and Lindholm, 2008 [PubMed 18068114]).[supplied by OMIM].
Product Categories/Family for anti-TCF25 antibody
References
Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010). Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010). Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009). Steen, H., et al. Biochem. Biophys. Res. Commun. 366(2):432-437(2008). Cai, Z., et al. Biochem. Biophys. Res. Commun. 343(3):973-981(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
76,667 Da
NCBI Official Full Name
transcription factor 25
NCBI Official Synonym Full Names
transcription factor 25 (basic helix-loop-helix)
NCBI Official Symbol
TCF25
NCBI Official Synonym Symbols
Hulp1; NULP1; FKSG26; PRO2620; hKIAA1049
NCBI Protein Information
transcription factor 25; NULP1; TCF-25; nuclear localized protein 1
UniProt Protein Name
Transcription factor 25
Protein Family
UniProt Gene Name
TCF25
UniProt Synonym Gene Names
KIAA1049; NULP1; TCF-25
UniProt Entry Name
TCF25_HUMAN
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Product Notes
The TCF25 tcf25 (Catalog #AAA6354481) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TCF25, NT (TCF25, KIAA1049, NULP1, Transcription factor 25, Nuclear localized protein 1) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TCF25 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TCF25 tcf25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCF25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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