Rabbit ADM Polyclonal Antibody | anti-ADM antibody

ADM Antibody (Center)

Cat. Num. AAA9207591

• Gene Names: ADM; AM; PAMP
• Reactivity: Human (Predicted Reactivity: Bovine)
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: ADM; Polyclonal Antibody; ADM Antibody (Center); Adrenomedullin; AM; Proadrenomedullin N-20 terminal peptide; ProAM N-terminal 20 peptide; PAMP; ProAM-N20; anti-ADM antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This ADM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-96 amino acids from the Central region of human ADM.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.25 (varies by lot)
• Sequence Positions: 69-96
• Sequence Length: 185

• Applicable Applications for anti-ADM antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of ADM Antibody (Center) in mouse lung tissue lysates (35ug/lane).ADM (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(ADM Antibody (Center) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ADM antibody

ADM, a hypotensive peptide found in human pheochromocytoma, consists of 52 amino acids, has 1 intramolecular disulfide bond, and shows a slight homology with the calcitonin gene-related peptide. It may function as a hormone in circulation control because it is found in blood in a considerable concentration. The precursor, called preproadrenomedullin, is 185 amino acids long. By RNA-blot analysis, human adrenomedullin mRNA was found to be highly expressed in several tissues. Genomic ADM DNA consists of 4 exons and 3 introns, with the 5-prime flanking region containing TATA, CAAT, and GC boxes.

Product Categories/Family for anti-ADM antibody

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal transduction

References

Kim, S.M., et al. FEBS Lett. 584(1):213-218(2010)
Oie, E., et al. Basic Res. Cardiol. 105(1):89-98(2010)
Nomura, I., et al. Regul. Pept. 158 (1-3), 127-131 (2009)

NCBI and Uniprot Product Information

• NCBI GI #: 4501945
• NCBI GeneID: 133
• NCBI Accession #: NP_001115.1
• NCBI GenBank Nucleotide #: NM_001124.2
• UniProt Accession #: P35318; Q6FGW2; B2R793; D3DQV3
• Molecular Weight: 20420
• NCBI Official Full Name: ADM
• NCBI Official Synonym Full Names: adrenomedullin
• NCBI Official Symbol: ADM
• NCBI Official Synonym Symbols: AM; PAMP
• NCBI Protein Information: ADM
• UniProt Protein Name: ADM
• Protein Family: ADM
• UniProt Gene Name: ADM
• UniProt Synonym Gene Names: AM; AM; PAMP; ProAM-N20
• UniProt Entry Name: ADML_HUMAN

NCBI Description

The protein encoded by this gene is a preprohormone which is cleaved to form two biologically active peptides, adrenomedullin and proadrenomedullin N-terminal 20 peptide. Adrenomedullin is a 52 aa peptide with several functions, including vasodilation, regulation of hormone secretion, promotion of angiogenesis, and antimicrobial activity. The antimicrobial activity is antibacterial, as the peptide has been shown to kill E. coli and S. aureus at low concentration. [provided by RefSeq, Aug 2014]

Uniprot Description

ADM: AM and PAMP are potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, am is diuretic and natriuretic, and both am and pamp inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels. Belongs to the adrenomedullin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: protein binding; adrenomedullin receptor binding; hormone activity; receptor binding

Biological Process: developmental growth; blood circulation; positive regulation of apoptosis; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; female pregnancy; positive regulation of vasodilation; signal transduction; response to insulin stimulus; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; cAMP-mediated signaling; cell-cell signaling; receptor internalization; positive regulation of cell proliferation; positive regulation of cAMP biosynthetic process; response to wounding; vasculogenesis; regulation of the force of heart contraction; aging; hormone secretion; progesterone biosynthetic process; organ regeneration; androgen metabolic process; neurite regeneration; positive regulation of heart rate; negative regulation of vasoconstriction; odontogenesis of dentine-containing teeth; response to starvation; positive regulation of angiogenesis; G-protein coupled receptor internalization; neural tube closure; negative regulation of vascular permeability; response to hypoxia; cAMP biosynthetic process; response to cold